The Effect Flavonoids Phaleria macrocarpa Fruit Extract on Thickness of Trabeculae, Cortex Ratio Femoral Bone and Aortic Intima-Media in Mice Menopause Model

Authors

  • R. A. Rahmawati Nurul Fadilah Master Program of Midwifery, Department of Midwifery, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia https://orcid.org/0009-0002-5506-141X
  • Ani Khoirinda Master Program of Midwifery, Department of Midwifery, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia
  • Sutrisno Sutrisno Department of Obstetric and Gynecology, Brawijaya University, Malang, East Java, Indonesia
  • Yahya Irwanto Department of Obstetric and Gynecology, Brawijaya University, Malang, East Java, Indonesia
  • Kenty Wantri Anita Department of Anatomical Pathology, Brawijaya University, Malang, East Java, Indonesia
  • R. A. Rose Khasana Dewi Department of Anatomical Pathology, Brawijaya University, Malang, East Java, Indonesia

DOI:

https://doi.org/10.31965/infokes.Vol22.Iss2.1553

Keywords:

Flavonoid, Phaleria macrocarpa, Menopause, Osteoporosis, Atherosclerosis

Abstract

A deficiency of the hormone estrogen at menopause can lead to an increased rate of the destruction of the bone tissue that leads to bone loss, which can lead to osteoporosis and impaired fat metabolism, which increases the risk of atherosclerosis. Phytoestrogens from flavonoid extract P. Macrocarpa, having effects similar to endogenous estrogens themselves, prevent osteoporosis and atherosclerosis in menopausal women. The purpose of this research is to assess the influence of flavonoids from P. Macrocarpa fruit extract on trabeculae cortex thickness, ratio of femoral bone, and aortic IMT (A-IMT) in a menopausal mouse model. The study was conducted in a true experimental-posttest-only control group design. Using 32 mice; namely KN (normal mice with no treatment), KP (OVX with no treatment), P1(OVX and given flavonoid 3.75 mg/mice/day), P2 (OVX and given flavonoid 7.5 mg/mice/day), P3(OVX and given flavonoid 11.25 mg/mice/day), P4 (OVX and given flavonoid 15 mg/mice/day), the treatment given within 14 days. Then the thickness of the trabeculae, cortex, and intima-media aorta with Hematoxylin-Eosin (HE) staining. In the trabeculae, cortex thickness ratio obtained KN results meaningfully dissimilar to the KP group and the P3 and P4 groups were meaningfully dissimilar from the KP. The A-BMI in KP is meaningfully dissimilar to P1, P2, P3 and P4. The conclusion of the study is flavonoid fruit extract P. Macrocarpa can increase the thickness ratio of trabeculae, and cortex femoral bone of mice menopausal model in groups P3, P4 and can decrease A-IMT starting in groups P1 to P4.              

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References

Ahmad, R., Mazlan, M. K. N., Aziz, A. F. A., Gazzali, A. M., Rawa, M. S. A., & Wahab, H. A. (2023). Phaleria macrocarpa (Scheff.) Boerl.: An updated review of pharmacological effects, toxicity studies, and separation techniques. Saudi Pharmaceutical Journal, 31(6), 874-888. https://doi.org/10.1016/j.jsps.2023.04.006 DOI: https://doi.org/10.1016/j.jsps.2023.04.006

Athiroh AS, N., & Permatasari, N. (2012). Mekanisme Kerja Benalu Teh pada Pembuluh Darah: Mechanism of Tea Mistletoe Action on Blood Vessels. Jurnal Kedokteran Brawijaya, 27(1), 1–7. https://doi.org/10.21776/ub.jkb.2012.027.01.1 DOI: https://doi.org/10.21776/ub.jkb.2012.027.01.1

Bacciottini, L., Falchetti, A., Pampaloni, B., Bartolini, E., Carossino, A. M., & Brandi, M. L. (2007). Phytoestrogens: food or drug. Clin Cases Miner Bone Metab, 4(2), 123-130.

Biason-Lauber, A., & Lang-Muritano, M. (2022). Estrogens: Two nuclear receptors, multiple possibilities. Molecular and Cellular Endocrinology, 554(November 2020), 111710. https://doi.org/10.1016/j.mce.2022.111710 DOI: https://doi.org/10.1016/j.mce.2022.111710

BPS, Bappenas dan UNFPA Indonesia. (2008). Proyeksi Penduduk Indonesia 2005-2025. Jakarta: BPS, Bappenas dan UNFPA Indonesia

de Villiers, T. J. (2023). Bone health and menopause: Osteoporosis prevention and treatment. Best Practice and Research: Clinical Endocrinology and Metabolism, 38(1), 101782. https://doi.org/10.1016/j.beem.2023.101782 DOI: https://doi.org/10.1016/j.beem.2023.101782

Dumanauw, J. M., Minggus, R. E., Rintjap, D. S., Rumagit, B., & Maramis, R. N. (2022). Efek Farmakologi Tanaman Mahkota Dewa ( Phaleria Macrocarpa ( Scheff .) Boerl ) ( Studi Literatur ). E-Prosding Seminar Nasional Poltekkes Kemenkes Manado, 1(2), 157–167.

Erizon, E., & Karani, Y. (2020). HDL dan Aterosklerosis. Human Care Journal, 5(4), 11–23. https://doi.org/10.32883/hcj.v5i4.851 DOI: https://doi.org/10.32883/hcj.v5i4.851

Fitriana, S., Andarini, S., Sutrisno, S., Nawangtantrini, G., & Maharani, M. (2023). Hepatotoxicity Oral Administration of Flavonoids Rich Extract from Phaleria Macrocarpha in Mice. Asian Journal of Health Research, 2(2), 36-41. https://doi.org/10.55561/ajhr.v2i2.109 DOI: https://doi.org/10.55561/ajhr.v2i2.109

Forslund, L. C., & Andersson, H. C. (2017). Phytoestrogens in foods on the Nordic market: A literature review on occurrence and levels. Nordic Council of Ministers. DOI: https://doi.org/10.6027/TN2017-541

Geng, Q., Gao, H., Yang, R., Guo, K., & Miao, D. (2019). Pyrroloquinoline Quinone Prevents Estrogen Deficiency-Induced Osteoporosis by Inhibiting Oxidative Stress and Osteocyte Senescence. International journal of biological sciences, 15(1), 58–68. https://doi.org/10.7150/ijbs.25783 DOI: https://doi.org/10.7150/ijbs.25783

Goo, Y. H. (2019). Cholesterol metabolism in atherosclerosis development. The Molecular Nutrition of Fats, 299–306. https://doi.org/10.1016/B978-0-12-811297-7.00023-8 DOI: https://doi.org/10.1016/B978-0-12-811297-7.00023-8

Gosset, A., Pouillès, J. M., & Trémollieres, F. (2021). Menopausal hormone therapy for the management of osteoporosis. Best Practice & Research Clinical Endocrinology & Metabolism, 35(6), 101551. https://doi.org/10.1016/j.beem.2021.101551 DOI: https://doi.org/10.1016/j.beem.2021.101551

Hasanah, M., Bahri, S., & Merta, I. W. (2020). Effect of Red Bean Extract (Phaseolus vulgaris, L) on the Development of Female Mice Eggs (Mus musculus) Balb/C strains. Jurnal Penelitian Pendidikan IPA, 6(2), 227-231. https://doi.org/10.29303/jppipa.v6i2.390 DOI: https://doi.org/10.29303/jppipa.v6i2.390

Hopper, S. E., Cuomo, F., Ferruzzi, J., Burris, N. S., Roccabianca, S., Humphrey, J. D., & Figueroa, C. A. (2021). Comparative Study of Human and Murine Aortic Biomechanics and Hemodynamics in Vascular Aging. Frontiers in Physiology, 12(October), 1–14. https://doi.org/10.3389/fphys.2021.746796 DOI: https://doi.org/10.3389/fphys.2021.746796

Huff, T., Boyd, B., & Jialal, I. (2021). Physiology, Cholesterol. Statpearls Publishing. Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK470561/

Javadifar, A., Rastgoo, S., Banach, M., Jamialahmadi, T., Johnston, T. P., & Sahebkar, A. (2021). Foam cells as therapeutic targets in atherosclerosis with a focus on the regulatory roles of non-coding RNAs. International Journal of Molecular Sciences, 22(5), 1–27. https://doi.org/10.3390/ijms22052529 DOI: https://doi.org/10.3390/ijms22052529

Jebari-Benslaiman, S., Galicia-García, U., Larrea-Sebal, A., Olaetxea, J. R., Alloza, I., Vandenbroeck, K., Benito-Vicente, A., & Martín, C. (2022). Pathophysiology of Atherosclerosis. International Journal of Molecular Sciences, 23(6), 1–38. https://doi.org/10.3390/ijms23063346 DOI: https://doi.org/10.3390/ijms23063346

Juwita, D. A., & Fatma, R. M. (2021). Effect of Propolis on Bone Quality and Cortical Bone Thickness of Ovariectomized Female Wistar White Rats as A Model for Osteoporosis. Pharmaceutical Sciences and Research, 8(3), 121–127. https://doi.org/10.7454/psr.v8i3.1214 DOI: https://doi.org/10.7454/psr.v8i3.1214

Khatana, C., Saini, N. K., Chakrabarti, S., Saini, V., Sharma, A., Saini, R. V., & Saini, A. K. (2020). Mechanistic insights into the oxidized low‐density lipoprotein‐induced atherosclerosis. Oxidative medicine and cellular longevity, 2020(1), 5245308. https://doi.org/10.1155/2020/5245308 DOI: https://doi.org/10.1155/2020/5245308

Khoudary, S. R., Venugopal, V., Manson, J. E., Brooks, M., Santoro, N., Black, D. M., Harman, M., Hodis, H. N., Brinton, E. A., Miller, V. M., Taylor, H. S., & Budoff, M. J. (2020). Heart Fat and Carotid Artery Atherosclerosis Progression in Recently Menopausal Women: Impact of Menopausal Hormone Therapy. The KEEPS Trial, 27(3), 255–262. https://doi.org/10.1097/GME.0000000000001472.Heart DOI: https://doi.org/10.1097/GME.0000000000001472

Kuhnle, G. G. C., Dell’Aquila, C., Aspinall, S. M., Runswick, S. A., Joosen, A. M. C. P., Mulligan, A. A., & Bingham, S. A. (2009). Phytoestrogen content of fruits and vegetables commonly consumed in the UK based on LC-MS and 13C-labelled standards. Food Chemistry, 116(2), 542–554. https://doi.org/10.1016/j.foodchem.2009.03.002 DOI: https://doi.org/10.1016/j.foodchem.2009.03.002

Liu, X. (2020). Crucial molecular mechanisms of phytoestrogen regulation in osteoporosis. Front Endocrinol (Lausanne), 11(1), 608–634. https://doi.org/10.3389/fendo.2020.608634

Macpherson, B. E., & Quinton, N. D. (2022). Menopause and healthcare professional education: A scoping review. Maturitas, 166(August), 89–95. https://doi.org/10.1016/j.maturitas.2022.08.009 DOI: https://doi.org/10.1016/j.maturitas.2022.08.009

Maharani, M., Lajuna, L., Yuniwati, C., Sabrida, O., & Sutrisno, S. (2021). Phytochemical characteristics from Phaleria macrocarpa and its inhibitory activity on the peritoneal damage of endometriosis. Journal of Ayurveda and Integrative Medicine, 12(2), 229–233. https://doi.org/10.1016/j.jaim.2020.06.002 DOI: https://doi.org/10.1016/j.jaim.2020.06.002

Maharani, & Sutrisno. (2021). Pengaruh Flavonoid Ekstrak Mahkota Dewa (Phaleria Macrocarpa) Terhadap Peningkatan Indeks Apoptosis Pada Peritoneal Mencit Model Endometriosis Abstract Effect of Flavonoid Extract From Mahkota Dewa (Phaleria Macrocarpa) on Increasing Cell Apoptotic Index . Jurnal Kebidanan Malahayati, 7(4), 652–657. https://doi.org/10.33024/jkm.v7i4.4606 DOI: https://doi.org/10.33024/jkm.v7i4.4606

Mirza, D. M., Purbosari, I., Hardjono, S., & Agil, M. (2021). Prediksi Aktivitas Fitoestrogenik Senyawa Golongan Flavonoid terhadap Receptor Estrogen α (ER- α) dengan pendekatan In Silico Prediction. Jurnal Sains Dan Kesehatan, 3(4), 512–519. DOI: https://doi.org/10.25026/jsk.v3i4.640

Nayak, T., Freaney, P. M., & Maganti, K. (2022). Atherosclerotic Cardiovascular Disease Risk Assessment and Menopause: Current Evidence. American College of Cardiology.

Newson, L. (2018). Menopause and cardiovascular disease. Post Reproductive Health, 24(1), 44–49. https://doi.org/10.1177/2053369117749675 DOI: https://doi.org/10.1177/2053369117749675

NIH. (2022). Structure of Bone Tissue. Seer Training Moduls Natioanal Institute of Health. Retrieved from https://training.seer.cancer.gov/anatomy/skeletal/tissue.html

Pratiwi, M., & Damayanty, A. E. (2020). Pengaruh Pemberian Susu Kedelai (Glicine Max L. Merr) terhadap Kadar HDL dan LDL pada Wanita Menopause (Studi pada Ibu-Ibu Pengajian Aisyiyah Ranting Melati Medan). JURNAL ILMIAH KOHESI, 4(4), 132-137. DOI: https://doi.org/10.30596/jih.v1i1.4540

Pujiastuti, E., & El’Zeba, D. (2021). Perbandingan Kadar Flavonoid Total Ekstrak Etanol 70% dan 96% Kulit Buah Naga Merah Hylocereus Polyrhizus) dengan Spektrofotometri. Cendekia Journal of Pharmacy, 5(1), 28–43. https://doi.org/10.31596/cjp.v5i1.131 DOI: https://doi.org/10.31596/cjp.v5i1.131

Rizalah, S., Hasan, M., & Wahyudi, S. S. (2016). Pengaruh Pemberian Kitosan Cangkang Udang Putih ( Penaeus merguiensis ) terhadap Ketebalan Trabekular Femur Tikus Wistar Betina Pasca Ovariektomi. E-Jurnal Pustaka Kesehatan, 4(1), 146–151.

Rochmah, K. (2008). Potensi Ekstrak Buah Mahkota Dewa (Phaleria macrocarpa) Sebagai Antioksidan Dalam Pengaturan Profil Lipid Darah Mencit. Jurnal Faal Indonesia, 7(3), 155–242.

Sadik, F., & Saiful Bachri, M. (2021). Uji Efektivitas Ekstrak Etanol Daun Jarak Pagar (Jatropha Curcas. L) Sebagai Antihipertensi Pada Tikus. Kieraha Medical Journal, 3(2), 2686–5912. https://doi.org/10.33387/kmj.v3i2.3949 DOI: https://doi.org/10.33387/kmj.v3i2.3949

Sargowo, D. (2015). Disfungsi Endotel. UB Press.

Silva, T. R., Oppermann, K., Reis, F. M., & Spritzer, P. M. (2021). Review nutrition in menopausal women: A narrative review. Nutrients, 13(7), 1–14. https://doi.org/10.3390/nu13072149 DOI: https://doi.org/10.3390/nu13072149

Stephenus, F. N., Benjamin, M. A. Z., Anuar, A., & Awang, M. A. (2023). Effect of Temperatures on Drying Kinetics, Extraction Yield, Phenolics, Flavonoids, and Antioxidant Activity of Phaleria macrocarpa (Scheff.) Boerl. (Mahkota Dewa) Fruits. Foods, 12(1), 1–19. https://doi.org/10.3390/foods12152859 DOI: https://doi.org/10.3390/foods12152859

WHO. (2022). Menopause. World Health Organization.

Women Health Concern. (2021). Osteoporosis – Bone Health Following the Menopause. Women Health Concern.

Yousefzadeh, N., Kashfi, K., Jeddi, S., & Ghasemi, A. (2020). Ovariectomized rat model of osteoporosis: A practical guide. EXCLI Journal, 19, 89–107. https://doi.org/10.17179/excli2019-1990

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Published

2024-06-30

How to Cite

Fadilah, R. A. R. N., Khoirinda, A., Sutrisno, S., Irwanto, Y., Anita, K. W., & Dewi, R. A. R. K. (2024). The Effect Flavonoids Phaleria macrocarpa Fruit Extract on Thickness of Trabeculae, Cortex Ratio Femoral Bone and Aortic Intima-Media in Mice Menopause Model. JURNAL INFO KESEHATAN, 22(2), 307–316. https://doi.org/10.31965/infokes.Vol22.Iss2.1553

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Vol. 22 No. 2 (2024): JURNAL INFO KESEHATAN

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