Peningkatan Polymorphonuclear (PMN) Dalam Cairan Nasal Lavage Operator Penggilingan Padi Yang Terpajan Endotoksin Lipopolisakarida (LPS)

Authors

DOI:

https://doi.org/10.31965/infokes.Vol16.Iss1.175

Keywords:

Polymorphonuclear, Endotoxin, Rice mills

Abstract

Background: Exposure to lipopolysaccharide endotoxin (LPS) in rice dust causes respiratory inflammation which is characterized by an increase in Neutrophils or Polymorphonuclear (PMN) in the nasal lavage fluid of rice milling operators. Objective: The purpose of this study was to analyze the effect of endotoxin LPS on the rice mill operators' PM in the rice level operators. Methods: the design of this study was a longitudinal study. Dust sampling was carried out for 8 hours while nasal lavage samples were carried out before and after work (cross shift). Endotoxin PMN and LPS were analyzed using the ELISA method and LPS Endotoxin was analyzed using LAL. Results: The mean level of LPS endotoxin in rice dust was 56.36 ± 5.83 EU / m3. An increase in post-employment PMN (Pired sample t-test: p = 0.000) in all rice milling operators. LPS endotoxin levels correlated with increased PMN in nasal lavage fluid in rice milling operators (multiple linear regression test: p = 0.000). Conclusion: LPS endotoxin is a factor that influences the increase of PMN levels in nasal lavage fluid which indicates the occurrence of inflammation in rice mill operators. Suggestion: Use a rice milling machine equipped with a dust collector. Health checks are given to the rest of their work. Workers are advised to always use masks while working.

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References

Antarudin, (2003). Pengaruh Debu Padi pada Faal Paru Pekerja Kilang Padi yang Merokok dan Tidak Merokok, Tesis Fakultas Kedokteran Universitas Sumatera Utara. http://www.repository.usu.ac.id/bitstream (Sitasi 29 November 2014).

Baratawidjaja KG., Rengganis I (2013). Imunologi Dasar. Balai Penerbit Fakultas Kedokteran Universitas Indonesia.

Baratawidjadja KG (2010). Imunologi Dasar. Edisi 8. Balai Penerbit Fakultas Kedokteran Universitas Indonesia. Jakarta

Becker S., Clapp W.A., Quai J., Fress K.L (1999). Compartmentalization of the inflammatory Respon to Inhaled Grain Dust. Am J Respir Crit Care Med, 160 (4); 1309-1318. http://www.ncbi.nlm.nih.gov/pmc/articels (sitasi 2 Desember 2014).

Clapp W D S., Becker J Q., Watt J L., Thorne P S., Frees K L., Zhang X., Koren H S., Lux C R., Schwartz D A (1994). Cytokine gene expression after inhalation of corn dust. American Journal of Respiratory and Critical Care Medicine (Impact Factor: 11.04). 10/1994; Source: PubMed. (sitasi 2 Desember 2014).

Djojodibroto R.D (2014). Respilogi. Penerbit Buku Kedokteran. Universitas Malaysia Sarawak.

Gezondheidsraad (2011). Grain Dust, Health Based Recommended Occupational Exposure Limit. http://www.gezondheidsraad.nl/sites (sitasi 25 November 2014).

Hermawan A.G. (2006). SIRS dan Spesis. Imunologi, Diagnosis, Penatalaksanaan. Surakarta: Sebelas Maret University Press.

Jancinova V., Perecko T., Nosal R., Mihalova D., Bauerova K., Drabikova K. (2011).Pharmacological regulation of neutrophil activity and apoptosis. Interdisc Toxicol. 2011; Vol. 4(1): 11–14. Published online in: http://www.intertox.sav.sk & http://www.versita.com/science/medicine/it/ (disitasi pada 23 April 2015)

Keman S (1997). Biomarkers of Chronic Non Spesific Airway Diseases – An Application of Molecular Epidemiology in Occupational Settings. Disertation. Netherlands: Maastricht University.

Keman S., Jetten, M., Douwes, J., Borm, P.J.A.(1998). Longitudinal Changes in Inflammatory Markers in Nasal Lavage of Cotton Workers: Relation to Endotoxin Exposure and Lung Function Changes. International Archive of Occupational and Environmental Health 71: 131-137.

Kennedy SM., Dimich-Ward H., Desjardin A., Kassam A., Vedal S., Chan-Yeung M (1994). Respiratory Health Among Retired Grain Elevator Workers.Am J Respir Crit Care Med 150: 59-65. http://www.ajplung.physiology.org. (sitasi 22 Februari 2015).

O’Grady N.P., Preas H.L., Pugin J. (2001) Local Inflammatory Responses Following Bronchial Enditoxin Instillation in Humans. American Journal of Respiratory and Critical Care Medicine. 163: 1591-1598

Poole J.A. (2012). Farming-Associated Environmental Exposures and Effect on Atopic Disease. Ann Allergy Atshma Immunol. 109:93-98 (PMC free article) (PubMed). (sitasi tanggal 20 Februari 2015).

Quinn MT., Gauss KA. (2004). Structure and Regulation of the Neutrophile Respiratory Burst Oxidase: Comparation With Nonphagocyte Oxidase. Journal of Leucocyte Biology. 76(4):900-914.

Schwartz DA., Landas SK., Lassise DL., Burmeistrer LF., Huninghake GW., Merchant JA (1992). Aiwway injury in swine confinement workers. Ann Intern Med. 116:360-5 (PubMed). (sitasi tanggal 25 Januari 2015).

Sirait, M. (2010). Hubungan Karakteristik Pekerja dengan Faal Paru di Kilang Padi Kecamatan Porsea tahun 2010. Skripsi. Fakultas Kesehatan Masyarakat Universitas Sumatera Utara, Medan.

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Published

2018-06-04

How to Cite

Lamawuran, W. W. (2018). Peningkatan Polymorphonuclear (PMN) Dalam Cairan Nasal Lavage Operator Penggilingan Padi Yang Terpajan Endotoksin Lipopolisakarida (LPS). JURNAL INFO KESEHATAN, 16(1), 96–105. https://doi.org/10.31965/infokes.Vol16.Iss1.175

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Original Articles