Anti-Cholesterolemic Activity of Mulberry (Morus australis Poir) Ethanol Extract in Increasing HDL Levels and Inhibiting Formation of Foam Cells on Rat

Authors

  • Priska Ernestina Tenda 1. Department of Pharmacy, Poltekkes Kemenkes Kupang, Kupang, East Nusa Tenggara, Indonesia
  • Eleonora Maryeta Toyo Nusaputera College of Pharmacy, Semarang, Central Java, Indonesia

DOI:

https://doi.org/10.31965/infokes.Vol19.Iss1.497

Keywords:

Ethanol Extract of Mulberry Leaves, HDL Level, Foam Cells

Abstract

Mulberry leaves are one of the medicinal plants empirically used by people to reduce cholesterol levels. Previous study found that mulberry leaves contain flavonoids, act as cholesterol-lowering and tannins function to inhibit the absorption of food, including fat in the GI tract. The objective of this study is to determine the effect of mulberry leaf ethanol extract on increased HDL levels and to determine the inhibition of the formation of foam cells in male Wistar strain rats given the atherogenic diet. Thirty male Wistar rats were divided into six groups, which were normal group, negative control, positive control, and three groups of mulberry leaf extract at a dose of 25, 50, and 100 mg/200g BW rats, respectively. The HDL levels were measured pre- and post- treatment on days 0, 28, 35 and 42. The results showed that the most effective dose augmenting the HDL as well as inhibited the formation of aortic wall foam cell was the ethanol extract at a dose of 100 mg/200 g BW. In conclusion, mulberry leaves ethanolic extract have the potency to be developed as a natural cholesterol-lowering agent.

Downloads

Download data is not yet available.

References

Amran, A. A., Zakaria, Z., Othman, F., Das, S., Al-Mekhlafi, H. M., & Nordin, N. A. M. (2011). Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental rabbits fed with cholesterol diet. Lipids in health and disease, 10(1), 1-8. doi:https://doi.org/10.1186/1476-511X-10-2 DOI: https://doi.org/10.1186/1476-511X-10-2

Chairungsi, N., Jumpatong, K., Phutdhawong, W., & Buddhasukh, D. (2006). Solvent effects in electrocoagulation of selected plant pigments and tannin. Molecules, 11(5), 309-317. doi: https://doi.org/10.3390/11050309 DOI: https://doi.org/10.3390/11050309

Dewi, Y. R., Santoso, L. M., & Tibrani, M. M. (2012). Pineapple (Ananascomosus (L.)Merr.)Juice activity test on total cholesterol and blood triglycerides of mice (Musmusculus L.) and their contribution to learning biology in high school. Skripsi. FKIP Sriwijaya University

Hariana, A. (2008). Tanaman Obat dan Khasiatnya. Jakarta: Cetakan V. Sel. Penebar Swadaya.

Hussain, M. A., Al Mamun, A., Peters, S. A., Woodward, M., & Huxley, R. R. (2016). The Burden of Cardiovascular Disease Attributable to Major Modifiable Risk Factors in Indonesia. Journal of epidemiology, 26(10), 515-521. doi: https://doi.org/10.2188/jea.JE20150178 DOI: https://doi.org/10.2188/jea.JE20150178

Japardi, I. (2002). Patomekanisme Stroke InfarkAterotrombotik. Medan: Fakultas Kedokteran , Universitas Sumatera Utara.

Jia, Y.-J., Liu, J., Guo, Y.-L., Xu, R.-X., Sun, J., & Li, J.-J. (2013). Dyslipidemia in rat fed with high-fat diet is not associated with PCSK9-LDL-receptor pathway but ageing. Journal of geriatric cardiology : JGC, 10(4), 361-368. doi:https://doi.org/10.3969/j.issn.1671-5411.2013.04.007

Lamanema, M. E. L. (2005). Perbandingan Profil Lipid Dan Perkembangan Lesi Aterosklerosis Pada Tikus Wistar Yang Diberi Diet Perasan Pare Dengan Diet Perasan Pare dan Statin (The Comparison of Lipid Profile and The Progression of Atherosclerotic Lesion between Momordica Charantia Juice Dietary with and without Statin on Wistar Rats). Tesis. Program Pasca Sarjana, Universitas Diponegoro Semarang.

Lenny, S. (2006). Senyawa Flavonoida, Fenilpropanoida dan Alkaloida. Skripsi. Medan: Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara.

Mills, S., & Bone, K. (2000). Principles and practice of phytotherapy. Modern Herbal Medicine. UK: Churchill Livingstone.

Murray, RK., D.K Granner, P.A. Mayes, dan V.W. Rodwell. (2008). Biokimia Harper. Edisi 25. Jakarta: Penerbit Buku Kedokteran EGC.

Ruel, G., Pomerleau, S., Couture, P., Lemieux, S., Lamarche, B., & Couillard, C. (2006). Favourable impact of low-calorie cranberry juice consumption on plasma HDL-cholesterol concentrations in men. British Journal of Nutrition, 96(2), 357-364. doi:https://doi.org/10.1079/BJN20061814 DOI: https://doi.org/10.1079/BJN20061814

Sawangjaroen, N., Sawangjaroen, K., & Poonpanang, P. (2004). Effects of Piper longum fruit, Piper sarmentosum root and Quercus infectoria nut gall on caecal amoebiasis in mice. Journal of Ethnopharmacology, 91(2-3), 357-360. doi:https://doi.org/10.1016/j.jep.2004.01.014 DOI: https://doi.org/10.1016/j.jep.2004.01.014

Smeriglio, A., Barreca, D., Bellocco, E., & Trombetta, D. (2017). Proanthocyanidins and hydrolysable tannins: occurrence, dietary intake and pharmacological effects. British journal of pharmacology, 174(11), 1244-1262. doi: https://doi.org/10.1111/bph.13630 DOI: https://doi.org/10.1111/bph.13630

Sutedjo, A. Y. (2006). Mengenal penyakit melalui hasil pemeriksaan laboratorium. Yogyakarta: Amara Books.

Syamsuhidayat, S. S., & Hutapea, J. R. (1991). Inventaris tanaman obat Indonesia. Jakarta: Departemen Kesehatan Republik Indonesia.

Uli, R. E., Satyana, R. P., Zomer, E., Magliano, D., Liew, D., & Ademi, Z. (2020). Health and productivity burden of coronary heart disease in the working Indonesian population using life-table modelling. BMJ open, 10(9), e039221. doi:http://dx.doi.org/10.1136/bmjopen-2020-039221 DOI: https://doi.org/10.1136/bmjopen-2020-039221

Valacchi, G., Belmonte, G., Miracco, C., Eo, H., & Lim, Y. (2014). Effect of combined mulberry leaf and fruit extract on liver and skin cholesterol transporters in high fat diet-induced obese mice. Nutrition research and practice, 8(1), 20-26. doi:https://doi.org/10.4162/nrp.2014.8.1.20 DOI: https://doi.org/10.4162/nrp.2014.8.1.20

Wierda, R. J., Geutskens, S. B., Jukema, J. W., Quax, P. H., & van den Elsen, P. J. (2010). Epigenetics in atherosclerosis and inflammation. Journal of cellular and molecular medicine, 14(6a), 1225-1240. doi: https://doi.org/10.1111/j.1582-4934.2010.01022.x DOI: https://doi.org/10.1111/j.1582-4934.2010.01022.x

Xu, S., Pelisek, J., & Jin, Z. G. (2018). Atherosclerosis is an epigenetic disease. Trends in Endocrinology & Metabolism, 29(11), 739-742. doi: https://doi.org/10.1016/j.tem.2018.04.007 DOI: https://doi.org/10.1016/j.tem.2018.04.007

Zeka, K., Ruparelia, K., Arroo, R. R., Budriesi, R., & Micucci, M. (2017). Flavonoids and their metabolites: prevention in cardiovascular diseases and diabetes. Diseases, 5(3), 19. doi:https://doi.org/10.3390/diseases5030019 DOI: https://doi.org/10.3390/diseases5030019

Zhang, X., Jin, Y., Wu, Y., Zhang, C., Jin, D., Zheng, Q., & Li, Y. (2018). Anti-hyperglycemic and anti-hyperlipidemia effects of the alkaloid-rich extract from barks of Litsea glutinosa in ob/ob mice. Scientific reports, 8(12646), 1-10. doi:https://doi.org/10.1038/s41598-018-30823-w DOI: https://doi.org/10.1038/s41598-018-30823-w

Downloads

Published

2021-06-30

How to Cite

Tenda, P. E., & Toyo, E. M. . (2021). Anti-Cholesterolemic Activity of Mulberry (Morus australis Poir) Ethanol Extract in Increasing HDL Levels and Inhibiting Formation of Foam Cells on Rat. JURNAL INFO KESEHATAN, 19(1), 77–84. https://doi.org/10.31965/infokes.Vol19.Iss1.497

Issue

Section

Original Articles

Similar Articles

<< < 2 3 4 5 6 7 8 9 10 11 > >> 

You may also start an advanced similarity search for this article.