The Assay of Blood Plasma’s Malondialdehyde (MDA) Activity in Alloxan-Induced Diabetic Rat Given Yellow Velvet Leaf Extract (Limnocharis flava)
DOI:
https://doi.org/10.31965/infokes.Vol18.Iss2.481Keywords:
Blood Plasma’s MDA, Yellow Velvet Leaf, AntioxidantsAbstract
It has been well-known that Genjer or yellow velvet leaf (Limnocharis flava) has a potential as an alternative source to natural antioxidants. Antioxidants, such as flavonoids, alkaloids, phenols, tannins, and others are substances which can block the presence of free radicals coming into the body. It has also been examined to be beneficial in treating metabolic syndrome diseases such as diabetes, as it improves insulin’s performance. The objective of this study is to examine the antioxidant effect of yellow velvet leaf’s ethanolic extract (Limnocharis Flava) on blood plasma’s MDA activity in alloxan-induced diabetic rats (Rattus novergicus) in three different doses. The testing animals were randomly divided into five groups, each group consisted of 6 white rats. Group 1 was the negative control and given CMC Na 0.5%, while group 2 was a positive control and given glibenclamide 0.45 mg, group 3 was yellow velvet leaf’s ethanolic extract of 32.5 mg/kg, group 4 was yellow velvet leaf’s ethanolic extract of 65 mg/kg, group 5 was yellow velvet leaf’s ethanolic extract of 130 mg/kg. Previously, all groups were induced with alloxan through intra peritoneal injection. Ethanol extract was provided once on the day of 7th, 14th, and 21st. The observed parameters were blood plasma’s MDA activity in the alloxan-induced diabetic rats (Rattus novergicus). Blood plasma’s MDA activities were assessed by using Thiobarbituric Acid Reactive Substance (TBARs) method. Then, data were collected and analyzed by using One Way ANOVA followed by a Post hoc test. The results showed that the mean values of MDA levels in the testing groups of 1,2,3,4 and 5 were 9.30 ± 0.462, 2.17 ± 0.121, 6.11 ± 0.381, 4.07 ± 0.327, and 2.75 ± 0.121, respectively. One Way ANOVA test showed a significant difference in the blood plasma’s MDA levels among the groups (p = 0,000). It is concluded that the blood plasma’s MDA levels in alloxan-induced diabetic rats can be lowered by the yellow velvet leaf’s ethanolic extract (Limnocharis Flava). Therefore, it can be employed as a traditional treatment for diabetes.
Downloads
References
Aprilia, N., Ariyani, A. D., & Hidayatin, N. (2018). The Effect Of Stew Beans To Blood Sugar Level In Diabetes Mellitus Patients. Jurnal Kesehatan, 11(2), 122-135. doi: https://doi.org/10.24252/kesehatan.v11i2.6454
Astuti, S. (2012). Isoflavon kedelai dan potensinya sebagai penangkap radikal bebas. Jurnal Teknologi & Industri Hasil Pertanian, 13(2), 126-136.
Bajaj, S., & Khan, A. (2012). Antioxidants and diabetes. Indian journal of endocrinology and metabolism, 16 (Suppl 2), S267–S271. doi: https://doi.org/10.4103/2230-8210.104057
Elgaml, S. A., & Hashish, E. A. (2014). Clinicopathological Studies Of Thymus Vulgaris Extractagainst Cadmium Induced Hepatotoxicity In Albino Rats. Global Journal of Pharmacology 8 (4): 501-509. doi: https://doi.org/ 10.5829/idosi.gjp.2014.8.4.8444
Juhaeti, T. (2013). Respon Genjer {Limnocharis flava (L.) Buchenau.} Terhadap Pemupukan dan Potensi Gizinya Untuk Diversifikasi Konsumsi Sayuran. Berita Biologi, 12(1), 107-116.
Khangholi, S., Majid, F. A. A., Berwary, N. J. A., Ahmad, F., & Abd Aziz, R. B. (2016). The mechanisms of inhibition of advanced glycation end products formation through polyphenols in hyperglycemic condition. Planta medica, 82(01/02), 32-45.
Monroy, M. L. L. D. L. V & Mejía, C. F. (2013). Oxidative stress in diabetes mellitus and the role of vitamins with antioxidant actions. Oxidative stress and chronic degenerative diseases-a role for antioxidants. London: Intech Open Limited. doi: https://doi.org/10.5772/51788
Narwanti, I., & Hamida, I. A. (2018). Uji Aktivitas Antioksidan Fraksi N-Heksana, Kloroform Dan Etil Asetat Ekstrak Etanol Limnocharis Flava Dengan Metode DPPH. Jurnal Insan Farmasi Indonesia, 1(2), 251-259.
Nowotny, K., Jung, T., Höhn, A., Weber, D., & Grune, T. (2015). Advanced glycation end products and oxidative stress in type 2 diabetes mellitus. Biomolecules, 5(1), 194-222. doi: https://doi.org/10.3390/biom5010194
Nurjanah, N., Jacoeb, A. M., Nugraha, R., Permatasari, M., & Sejati, T. K. A. (2014). Perubahan komposisi kimia, aktivitas antioksidan, vitamin C dan mineral tanaman genjer (Limnocharis flava) akibat pengukusan. Asian Journal of Innovation and Entrepreneurship, 3(3), 185-195. doi: https://doi.org/10.20885/ajie.vol3.iss3.art3
Oyenihi, A. B., Ayeleso, A. O., Mukwevho, E., & Masola, B. (2015). Antioxidant strategies in the management of diabetic neuropathy. Biomed Res Int, 2014(515042), 1-15.
Prawitasari, D. S. (2019). Diabetes Melitus dan Antioksidan. KELUWIH: Jurnal Kesehatan dan Kedokteran, 1(1), 48-52. doi: https://doi.org/10.24123/kesdok.V1i1.2496.
Prasadhana, E. R., Arumsari, A., & Kurniaty, N. (2019). Uji Aktivitas Antibakteri Ekstrak Etanol Tanaman Genjer (Limnocharis Flava (L.) Buchenau) Terhadap Bakteri Escherichia Coli dan Bacillus Subtilis. Skripsi. Bandung: Program Studi Farmasi, Universitas Islam Bandung.
Rajendiran, D., Packirisamy, S., & Gunasekaran, K. (2018). A Review on Role of Antioxidants in Diabetes. Asian Journal of Pharmaceutical and Clinical Research, 11(2), 48-53. doi: https://doi.org/10.22159/ajpcr.2018.v11i2.23241
Situmorang, N., & Zulham, Z. (2020). Malondialdehyde (MDA)(Zat Oksidan Yang Mempercepat Proses Penuaan). Jurnal Keperawatan Dan Fisioterapi (JKF), 2(2), 117-123. doi: https://doi.org/10.35451/jkf.v2i2.338
Widyawati, P. S. (2016). Determination of antixidant capacity in Pluchea indica Less leaves extract and its frantions. International Journal of Pharmacy and Pharmaceutical Sciences, 8(9), 32-36. doi: https://doi.org/10.22159/ijpps.2016v8i9.11410
Widyaningsih, W., Sativa, R., & Primardiana, I. (2015). Efek antioksidan ekstrak etanol ganggang hijau (Ulva lactuca L.) terhadap kadar malondialdehid (MDA) dan aktivitas enzim superoksida dismutase (SOD) hepar tikus yang diinduksi CCL4. Media Farmasi, 12(2), 163-175.
Yuda, I., Anthara, M., & Dharmayudha, A. (2013). Identifikasi Golongan Senyawa Kimia Estrak Etanol Buah Pare (Momordica charantia) dan Pengaruhnya Terhadap Penurunan Kadar Glukosa Darah Tikus Putih Jantan (Rattus novergicus) yang Diinduksi Aloksan. Buletin Veteriner Udayana, . Retrieved from https://ojs.unud.ac.id/index.php/buletinvet/article/view/7193
Zaetun, S., Dewi, L. B. K., Wiadnya, I. B. R., & Gede, L. S. (2019). Profil Kadar Mda (Malondialdehide) Sebagai Penanda Kerusakan Seluler Akibat Radikal Bebas Pada Tikus Yang Diberikan Air Beroksigen. Jurnal Analis Medika Biosains (JAMBS), 4(2), 63-68.
Zakaria, F. R., Prangdimurti, E., Puspawati, G. K. D., Thahir, R., & Suismono, S. (2011). Diet Berbasis Sorgum (Sorghum bicolor L Moench) Memperbaiki Proliferasi Limfosit Limfa dan Kapasitas Antioksidan Hati Tikus. Jurnal Pangan, 20(3), 209-222.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 JURNAL INFO KESEHATAN
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Copyright notice
Ownership of copyright
The copyright in this website and the material on this website (including without limitation the text, computer code, artwork, photographs, images, music, audio material, video material and audio-visual material on this website) is owned by JURNAL INFO KESEHATAN and its licensors.
Copyright license
JURNAL INFO KESEHATAN grants to you a worldwide non-exclusive royalty-free revocable license to:
- view this website and the material on this website on a computer or mobile device via a web browser;
- copy and store this website and the material on this website in your web browser cache memory; and
- print pages from this website for your use.
- All articles published by JURNAL INFO KESEHATAN are licensed under the Creative Commons Attribution 4.0 International License. This permits anyone to copy, redistribute, remix, transmit and adapt the work provided the original work and source is appropriately cited.
JURNAL INFO KESEHATAN does not grant you any other rights in relation to this website or the material on this website. In other words, all other rights are reserved.
For the avoidance of doubt, you must not adapt, edit, change, transform, publish, republish, distribute, redistribute, broadcast, rebroadcast or show or play in public this website or the material on this website (in any form or media) without appropriately and conspicuously citing the original work and source or JURNAL INFO KESEHATAN prior written permission.
Permissions
You may request permission to use the copyright materials on this website by writing to jurnalinfokesehatan@gmail.com.
Enforcement of copyright
JURNAL INFO KESEHATAN takes the protection of its copyright very seriously.
If JURNAL INFO KESEHATAN discovers that you have used its copyright materials in contravention of the license above, JURNAL INFO KESEHATAN may bring legal proceedings against you seeking monetary damages and an injunction to stop you using those materials. You could also be ordered to pay legal costs.
If you become aware of any use of JURNAL INFO KESEHATAN copyright materials that contravenes or may contravene the license above, please report this by email to jurnalinfokesehatan@gmail.com
Infringing material
If you become aware of any material on the website that you believe infringes your or any other person's copyright, please report this by email to jurnalinfokesehatan@gmail.com.
