Utilization of Teak Leaf Extract as a Substitute for Eosin in Hematoxylin Eosin Staining

Authors

  • Agnes Desiana Paa Poltekkes Kemenkes Kupang
  • Agustina W. Djuma Poltekkes Kemenkes Kupang
  • Juliana Christyaningsih Poltekkes Kemenkes Surabaya

Keywords:

Teak leaves, Eosin, Uterine tissue

Abstract

Hematoxylin–Eosin (HE) staining is widely used in histopathology to visualize tissue morphology. Eosin, a synthetic dye commonly used to stain cytoplasmic components, may cause environmental and health concerns, encouraging the search for safer natural alternatives. Young teak leaves (Tectona grandis) contain anthocyanin pigments with potential as natural staining agents. This study evaluated the effectiveness of teak leaf extract as a substitute for eosin in HE staining of uterine tissue. A quasi-experimental post-test only control group design was applied. Young teak leaves were extracted through maceration using 95% ethanol for seven days, followed by filtration and evaporation to obtain a concentrated extract. Uterine tissue preparations were stained using hematoxylin combined with 75% teak leaf extract, while eosin staining served as the control. Microscopic evaluation at 400x magnification was performed by an anatomical pathology specialist using a staining quality scoring system. The control group stained with eosin demonstrated optimal staining quality, with clear bluish-purple nuclei and bright pink cytoplasm, achieving a good score in all slides. In comparison, teak leaf extract produced pale purple nuclei and brownish-pink cytoplasm that remained distinguishable morphologically, though with lower staining quality. Overall, teak leaf extract demonstrated potential as a natural alternative to eosin, but further optimization is needed to improve staining performance.

References

Dibal, N. I., Garba, S. H., & Jacks, T. W. (2022). Histological Stains and Their Application in Teaching and Research. Asian Journal of Health Sciences, 8(2), ID43-ID43. 10.15419/ajhs.v8i2.514

Fathinatullabibah, F., Khasanah, L. U., & Kawiji, K. (2014). Stabilitas Antosianin Ekstrak Daun Jati (Tectona grandis) terhadap Perlakuan pH dan Suhu. Jurnal Aplikasi Teknologi Pangan, 3(2). https://garuda.kemdiktisaintek.go.id/documents/detail/699315

Fitrianingsih, A. (2022). Morfologi, Taksonomi dan Filosofi Tumbuhan. Penerbit P4I. Retrived from: https://books.google.co.id/books?id=QyWbEAAAQBAJ&printsec=copyright&hl=id#v=onepage&q&f=false

Golberg, M., Kobos, J., Clarke, E., Bajaka, A., Smędra, A., Balawender, K., ... & Żytkowski, A. (2024). Application of Histochemical Stains in Anatomical Research: a Brief Overview of the Methods. Translational Research in Anatomy, 35, 100294. https://doi.org/10.1016/j.tria.2024.100294

Gómez, S., Lafiosca, P., & Giovannini, T. (2024). Modeling UV/Vis Absorption Spectra of Food Colorants in Solution: Anthocyanins and Curcumin as Case Studies. Molecules, 29(18), 4378. https://doi.org/10.3390/molecules29184378

Haliza, N., Sari, I., Hartati, D., & Kurniawati, N. (2025). Pemanfaatan Ekstrak Bunga Asoka sebagai Alternatif Pengganti Eosin pada Pewarnaan Hematoxylin Eosin. Jurnal Riset Kesehatan Poltekkes Depkes Bandung Учредители: Politeknik Kesehatan Kemenkes Bandung, 17(1), 100-106. https://doi.org/10.34011/juriskesbdg.v17i1.2707

Hidayat, M. S., & Dewi, Y. R. (2024). Identifikasi Human Papillomavirus pada Wanita dengan Risiko Tinggi di Jakarta. Plenary Health: Jurnal Kesehatan Paripurna, 1(3), 297-301. https://doi.org/10.37985/plenaryhealth.v1i3.604

Imran, M. T., Shafi, I., Ahmad, J., Butt, M. F. U., Villar, S. G., Villena, E. G., ... & Ashraf, I. (2024). Virtual Histopathology Methods in Medical Imaging-a Systematic Review. BMC medical imaging, 24(1), 318. https://doi.org/10.1186/s12880-024-01498-9

Jumardi, M., Iswara, A., Putri, G. S. A., & Ariyadi, T. (2023). Perbandingan Kualitas Hasil Pewarnaan Menggunakan Hematoxylin Eosin dan Ekstrak Daun Jati sebagai Pengganti Eosin. Prosiding Seminar Nasional Unimus, 6. https://prosiding.unimus.ac.id/index.php/semnas/article/view/1582

Kembaren, R. Br., Putriliniar, S., Maulana, N. N., Yulianto, K., Ikono, R., Rochman, N. T., & Mardliyati, E. (2014). Ekstraksi dan Karakterisasi Serbuk Nano Pigmen dari Daun Tanaman Jati (Tectona grandis Linn. F). Jurnal Kimia dan Kemasan, 36(1), 191-196. 10.24817/jkk.v36i1.1904

Labai, D. M. (2023). Hasil Pewarnaan Sediaan Sitologi Metode Diff Quik Menggunakan Ekstrak Daun Jati (Tectona grandis) sebagai Pengganti Eosin. (D3) karya tulis ilmiah, Poltekkes Kemenkes Kupang.

Liyanda, N. J., & Kristanto, H. (2017). Diameter Arteri Spiralis Endometrium Mencit Balb/C yang Diberi Ekstrak Kunyit Asam (Curcuma domestica dan Tamarindus indica) secara Oral dalam Periode Gestasi dengan Dosis Bertingkat. Jurnal Kedokteran Diponegoro, 6(2), 893-899. https://doi.org/10.14710/dmj.v6i2.18599

Lowe, J. S., Anderson, P. G., & Anderson, S. I. (2023). Stevens & Lowe's Human Histology-E-Book: Stevens & Lowe's Human Histology-E-Book. Elsevier Health Sciences. Retrieved from: https://books.google.co.id/books?id=xZrxEAAAQBAJ&printsec=frontcover&source=gbs_ViewAPI&redir_esc=y#v=onepage&q&f=false

Marques, J. P. R., & Soares, M. K. M. (2022). Handbook of Techniques in Plant Histopathology. Cham, Switzerland, CH: Springer International Publishing. Retrieved from: https://link.springer.com/book/10.1007/978-3-031-14659-6

Marques, M. C., & Mariutti, L. R. B. (2025). Analysis of Anthocyanins: Extraction, Quantification, and Identification. In Food Chemistry: Basic Protocols (pp. 353-368). New York, NY: Springer US. Retrieved from: https://doi.org/10.1007/978-1-0716-4678-6_25

Mutoharoh, L., Santoso, S. D., & Mandasari, A. A. (2020). Pemanfaatan Ekstrak Bunga Sepatu (Hibiscus rosa-sinensis L.) sebagai Alternatif Pewarna Alami Sediaan Sitologi Pengganti Eosin pada Pengecatan Diff Quik. Jurnal SainHealth, 4(2), 21-26. https://doi.org/10.51804/jsh.v4i2.770.21-26

Nadhira, F. F., Rahmat, M., Mulia, Y. S., & Rismiarti, Z. (2023). Ekstrak Daun Jati (Tectona grandis) sebagai Alternatif Pengganti Eosin dalam Pemeriksaan Telur Cacing Golongan Soil Transmitted Helminths. Jurnal Kesehatan Siliwangi, 4(1), 165-171. https://doi.org/10.34011/jks.v4i1.1502

Nadifah, F., Prasetyaningsih, Y., Muhajir, N. F., & Della Puspita, E. (2024). Potensi Ekstrak Kulit Manggis (Garcinia mangostana L.) sebagai Alternatif Pengganti Eosin pada Pewarnaan Hematoksilin-Eosin. Prosiding Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia, 3, 483-492. https://prosiding.aiptlmi-iasmlt.id/index.php/prosiding/article/view/346

Rahmawanti, A., Setyowati, D. N., & Mukhlis, A. (2021). Histopathological of Brain, Eye, Liver, Spleen Organs of Grouper Suspected VNN in Penyambuan Village, North Lombok. Jurnal Biologi Tropis, 21(1), 140-148. 10.29303/jbt.v21i1.2439

Sari, Y. E. S., & Hariyanto, H. (2020). Rendaman Kuncup Daun Jati (Tectona grandis) Sebagai Alternative Pewarna Eosin pada Proses Histoteknik. PROSIDING SENAKES 1.0, 1(1).

Utami, Y. P., Fahria, F., Farizal, J., Hasanuddin, P., Mayasari, D., Pangaribuan, C., ... & Susana, Y. (2025). Teknik Dasar Histopatologi. CV Eureka Media Aksara. Retrieved from: https://books.google.co.id/books?hl=id&lr=&id=-kWyEQAAQBAJ&oi=fnd&pg=PA1&dq=Utami+,+Yuri+Pratiwi,+et+al+.+(+2023+).+Basic+Histopathology+Techniques+.+In+Cv.+Eureka+Media+Aksara+.+Purbalingga.+&ots=Vy_7aX2OWI&sig=WpYDC2IyxQ7eC-AYLRrNtRI-A8o&redir_esc=y#v=onepage&q&f=false

Downloads

Published

2026-06-30

How to Cite

Paa, A. D., Djuma, A. W., & Christyaningsih , J. (2026). Utilization of Teak Leaf Extract as a Substitute for Eosin in Hematoxylin Eosin Staining. Laboratory Journal of Infectious Diseases, 2(1), 8–15. Retrieved from https://jurnal.poltekkeskupang.ac.id/index.php/LJID/article/view/2419

Issue

Vol. 2 No. 1 (2026): Laboratory Journal of Infectious Diseases

Section

Articles

License

Copyright (c) 2026 Laboratory Journal of Infectious Diseases

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.