The Effects of Black Garlic on Ovarian Malondialdehyde, Oviduct Muscle, and Endometrial Arterioles in Smoke-Exposed Rats

Authors

  • Noni Fidya Ayu Anandasari Master Program of Midwifery, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
  • Novalia Kridayanti Master Program of Midwifery, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
  • Ni Ketut Devy Kaspirayanthi Master Program of Midwifery, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
  • Tatit Nurseta Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
  • Tri Yudani Mardining Raras Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Husnul Khotimah Faculty of Medical, Universitas Brawijaya, Malang, East Java, Indonesia
  • Subandi Reksohusodo Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
  • Kenty Wantri Anita Department of Pathological Anatomy, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
  • Hendy Setyo Yudhanto Department of Pathological Anatomy, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia

DOI:

https://doi.org/10.31965/infokes.Vol22.Iss2.1533

Keywords:

Black Garlic Extract, Cigarette Smoke, Oviduct Muscle, Malondialdehyde Levels, Endometrial Arterioles

Abstract

The effect of cigarette smoke exposure on reproductive health has been widely studied, showing various physiological disorders induced by free radicals and oxidative stress. This study aimed to assess the protective effect of ethanol extract of black garlic (Allium sativum) on some reproductive parameters of female rats Rattus norvegicus exposed to subacute cigarette smoke. A proper experimental method with a post-test-only control group design was used, involving 25 rats divided into five groups with three different doses of the extract. The rats were exposed to cigarette smoke and then treated for 28 days. Results showed a significant decrease in ovarian Malondialdehyde levels and increased fallopian tube smooth muscle thickness in the extract-treated group. In addition, black garlic extract successfully increased the number of endometrial arterioles in the group exposed to cigarette smoke and extract, in contrast to the group exposed to cigarette smoke only. This study concludes that black garlic extract has the potential to be a protective agent against oxidative damage in the reproductive system of rats exposed to cigarette smoke, with effectiveness that depends on the dose of extract given. Further studies should investigate the long-term effects and optimal dosing of black garlic extract in humans. Additionally, exploring the molecular mechanisms behind its protective properties could enhance its application in reproductive health management.                 

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References

Agustina, E., Andiarna, F., & Hidayati, I. (2020). Uji Aktivitas Antioksidan Ekstrak Bawang Hitam (Black Garlic) Dengan Variasi Lama Pemanasan. Al-Kauniyah: Jurnal Biologi, 13(1), 39–50. https://doi.org/10.15408/kauniyah.v13i1.12114 DOI: https://doi.org/10.15408/kauniyah.v13i1.12114

Amor, S., González-Hedström, D., Martín-Carro, B., Inarejos-García, A. M., Almodóvar, P., Prodanov, M., García-Villalón, A. L., & García, M. G. (2019). Beneficial effects of an aged black garlic extract in the metabolic and vascular alterations induced by a high fat/sucrose diet in male rats. Nutrients, 11(1). https://doi.org/10.3390/nu11010153 DOI: https://doi.org/10.3390/nu11010153

Aningsih, B. S. D., Sujuti, H., Mustofa, E., & Ratnawati, R. (2020). Anthocyanins from Ipomoea batatas L. Effect on ovarian malondialdehyde and 17β-estradiol in rats exposed cigarette smoke. Russian Open Medical Journal, 9(3), 1–5. https://doi.org/10.15275/rusomj.2020.0308 DOI: https://doi.org/10.15275/rusomj.2020.0308

Ardiana, M. (2021). Telaah Ilmiah Dan Patologi Paparan Asap Rokok Terhadap Penyakit Jantung. Airlangga University Press.

Budani, M. C., Carletti, E., & Tiboni, G. M. (2021). In Vivo Cigarette Smoke Exposure to Examine the Expression of Genes Involved in the Inflammatory Response in the Mouse Uterus. Current Protocols, 1(6), 1–9. https://doi.org/10.1002/cpz1.172 DOI: https://doi.org/10.1002/cpz1.172

Dampati, P. S., & Veronica, E. (2020). Potensi Ekstrak Bawang Hitam sebagai Tabir Surya terhadap Paparan Sinar Ultraviolet. KELUWIH: Jurnal Kesehatan Dan Kedokteran, 2(1), 23–31. https://doi.org/10.24123/kesdok.v2i1.3020 DOI: https://doi.org/10.24123/kesdok.V2i1.3020

Delbandi, A. A., Mahmoudi, M., Shervin, A., Heidari, S., Kolahdouz-Mohammadi, R., & Zarnani, A. H. (2020). Evaluation of apoptosis and angiogenesis in ectopic and eutopic stromal cells of patients with endometriosis compared to non-endometriotic controls. BMC Women’s Health, 20(1), 1–9. https://doi.org/10.1186/s12905-019-0865-4 DOI: https://doi.org/10.1186/s12905-019-0865-4

Djedjibegovic, J., Marjanovic, A., Panieri, E., & Saso, L. (2020). Ellagic Acid-Derived Urolithins as Modulators of Oxidative Stress. Oxidative Medicine and Cellular Longevity, 2020(Figure 1). https://doi.org/10.1155/2020/5194508 DOI: https://doi.org/10.1155/2020/5194508

Dutta, A., Dahiya, A., Prakash, A., & Agrawala, P. K. (2021). Acute toxicity of diallyl sulfide derived from Allium sativum (garlic) in mice and its possible mechanisms. Phytomedicine Plus, 1(3), 100084. https://doi.org/10.1016/j.phyplu.2021.100084 DOI: https://doi.org/10.1016/j.phyplu.2021.100084

Faber, M. T., Kjær, S. K., Dehlendorff, C., Chang-Claude, J., Andersen, K. K., Høgdall, E., Webb, P. M., Jordan, S. J., Rossing, M. A., Doherty, J. A., Lurie, G., Thompson, P. J., Carney, M. E., Goodman, M. T., Ness, R. B., Modugno, F., Edwards, R. P., Bunker, C. H., Goode, E. L., … Jensen, A. (2013). Cigarette smoking and risk of ovarian cancer: A pooled analysis of 21 case-control studies. Cancer Causes and Control, 24(5), 989–1004. https://doi.org/10.1007/s10552-013-0174-4 DOI: https://doi.org/10.1007/s10552-013-0174-4

Felix, A. S., Yang, H. P., Gierach, G. L., Park, Y., & Brinton, L. A. (2014). Cigarette smoking and endometrial carcinoma risk: The role of effect modification and tumor heterogeneity. Cancer Causes and Control, 25(4), 479–489. https://doi.org/10.1007/s10552-014-0350-1 DOI: https://doi.org/10.1007/s10552-014-0350-1

Gannon, A. M., Stämpfli, M. R., & Foster, W. G. (2013). Cigarette smoke exposure elicits increased autophagy and dysregulation of mitochondrial dynamics in murine granulosa cells. Biology of Reproduction, 88(3), 1–11. https://doi.org/10.1095/biolreprod.112.106617 DOI: https://doi.org/10.1095/biolreprod.112.106617

GATS. (2021). Gats|Global Adult Tobacco Survey Fact Sheet Indonesia 2021 Gats Objectives. Fact Sheet Indonesia, 1–2.

Gorrini, C., Harris, I. S., & Mak, T. W. (2013). Modulation of oxidative stress as an anticancer strategy. Nature Reviews Drug Discovery, 12(12), 931–947. https://doi.org/10.1038/nrd4002 DOI: https://doi.org/10.1038/nrd4002

Guo, X., Yi, H., Li, T. C., Wang, Y., Wang, H., & Chen, X. (2021). Role of vascular endothelial growth factor (Vegf) in human embryo implantation: Clinical implications. Biomolecules, 11(2), 1–16. https://doi.org/10.3390/biom11020253 DOI: https://doi.org/10.3390/biom11020253

Holbrook, B. D. (2016). The effects of nicotine on human fetal development. Birth Defects Research Part C - Embryo Today: Reviews, 108(2), 181–192. https://doi.org/10.1002/bdrc.21128 DOI: https://doi.org/10.1002/bdrc.21128

Hoyt, J. C., Robbins, R. A., Habib, M., Springall, D. R., Buttery, L. D. K., Polak, J. M., & Barnes, P. J. (2003). Cigarette smoke decreases inducible nitric oxide synthase in lung epithelial cells. Experimental Lung Research, 29(1), 17–28. https://doi.org/10.1080/01902140303759 DOI: https://doi.org/10.1080/01902140303759

Kida, N., Nishigaki, A., Kakita-Kobayashi, M., Tsubokura, H., Hashimoto, Y., Yoshida, A., Hisamatsu, Y., Tsuzuki-Nakao, T., Murata, H., & Okada, H. (2021). Exposure to cigarette smoke affects endometrial maturation including angiogenesis and decidualization. Reproductive Medicine and Biology, 20(1), 108–118. https://doi.org/10.1002/rmb2.12360 DOI: https://doi.org/10.1002/rmb2.12360

Kimura, S., Tung, Y. C., Pan, M. H., Su, N. W., Lai, Y. J., & Cheng, K. C. (2017). Black garlic: A critical review of its production, bioactivity, and application. Journal of Food and Drug Analysis, 25(1), 62–70. https://doi.org/10.1016/j.jfda.2016.11.003 DOI: https://doi.org/10.1016/j.jfda.2016.11.003

Kole, E., Ozkan, S. O., Eraldemir, C., Akar, F. Y., Ozbek, S. K., Kole, M. C., Kum, T., & Filiz, P. C. (2020). Effects of melatonin on ovarian reserve in cigarette smoking: An experimental study. Archives of Medical Science, 16(6), 1376–1386. https://doi.org/10.5114/AOMS.2019.89409 DOI: https://doi.org/10.5114/aoms.2019.89409

Lee, H. M., Kim, C. W., Hwang, K. A., Sung, J. H., Lee, J. K., & Choi, K. C. (2017). Cigarette smoke impaired maturation of ovarian follicles and normal growth of uterus inner wall of female wild-type and hypertensive rats. Reproductive Toxicology, 73, 232–240. https://doi.org/10.1016/j.reprotox.2017.06.187 DOI: https://doi.org/10.1016/j.reprotox.2017.06.187

Lu, X., Li, N., Qiao, X., Qiu, Z., & Liu, P. (2017). Composition analysis and antioxidant properties of black garlic extract. Journal of Food and Drug Analysis, 25(2), 340–349. https://doi.org/10.1016/j.jfda.2016.05.011 DOI: https://doi.org/10.1016/j.jfda.2016.05.011

Marengo, B., Nitti, M., Furfaro, A. L., Colla, R., Ciucis, C. De, Marinari, U. M., Pronzato, M. A., Traverso, N., & Domenicotti, C. (2016). Redox homeostasis and cellular antioxidant systems: Crucial players in cancer growth and therapy. Oxidative Medicine and Cellular Longevity, 2016. https://doi.org/10.1155/2016/6235641 DOI: https://doi.org/10.1155/2016/6235641

Ningrum, A. G., Frety, E. E., Diah, I., Shabran, Z. H., Setiani, R. E., & Dewi, E. R. (2021). Antioxidant activity of purslane (Portulaca oleracea l.) leaf extract on the levels of ovarian oxidative stress and reproductive hormone in rattus norvegicus exposed to cigarette smoke. Open Access Macedonian Journal of Medical Sciences, 9(B), 1535–1540. https://doi.org/10.3889/oamjms.2021.7081 DOI: https://doi.org/10.3889/oamjms.2021.7081

Oktari, Kwartika, Azizah, Z., Chandra, B., & Asra, R. (2020). A Review: Antioxidant and Immunomodulator Effects of Black Garlic. EAS Journal of Pharmacy and Pharmacology, 2(6), 193–198. https://doi.org/10.36349/easjpp.2020.v02i06.001 DOI: https://doi.org/10.36349/easjpp.2020.v02i06.001

Park, S. R., Kim, S. K., Kim, S. R., Yu, W. J., Lee, S. J., & Lee, H. Y. (2022). Effects of smoking on the tissue regeneration-associated functions of human endometrial stem cells via a novel target gene SERPINB2. Stem Cell Research and Therapy, 13(1), 1–20. https://doi.org/10.1186/s13287-022-03061-1 DOI: https://doi.org/10.1186/s13287-022-03061-1

Penke, B., Fulop, L., Szucs, M., & Frecska, E. (2017). The Role of Sigma-1 Receptor, an Intracellular Chaperone in Neurodegenerative Diseases. Current Neuropharmacology, 16(1), 97–116. https://doi.org/10.2174/1570159x15666170529104323 DOI: https://doi.org/10.2174/1570159X15666170529104323

Rahma, F., Ardiaria, M., & Panunggal, B. (2019). Pengaruh Pemberian Ubi Jalar Ungu (Ipomoea batatas l. poir) Terhadap Kadar Leukosit Total Tikus Wistar Jantan (Rattus norvegicus) Yang Dipapar Asap Rokok. Journal of Nutrition College, 8(2), 65. https://doi.org/10.14710/jnc.v8i2.23815 DOI: https://doi.org/10.14710/jnc.v8i2.23815

Recinella, L., Chiavaroli, A., Masciulli, F., Fraschetti, C., Filippi, A., Cesa, S., Cairone, F., Gorica, E., De Leo, M., Braca, A., Martelli, A., Calderone, V., Orlando, G., Ferrante, C., Menghini, L., Di Simone, S. C., Veschi, S., Cama, A., Brunetti, L., & Leone, S. (2021). Protective effects induced by a hydroalcoholic allium sativum extract in isolated mouse heart. Nutrients, 13(7). https://doi.org/10.3390/nu13072332 DOI: https://doi.org/10.3390/nu13072332

Sala, M., & Gotti, C. (2023). Electronic nicotine delivery systems (ENDS): A convenient means of smoking? Pharmacological Research, 195(June). https://doi.org/10.1016/j.phrs.2023.106885 DOI: https://doi.org/10.1016/j.phrs.2023.106885

Szumilas, K., Szumilas, P., Grzywacz, A., & Wilk, A. (2020). The effects of e-cigarette vapor components on the morphology and function of the male and female reproductive systems: A systematic review. International Journal of Environmental Research and Public Health, 17(17), 1–13. https://doi.org/10.3390/ijerph17176152 DOI: https://doi.org/10.3390/ijerph17176152

Tobacco Atlas. (2024). Findings from the Third Tobacconomics Cigarette Tax Scorecard. https://tobaccoatlas.org/inadequate-tobacco-tax-policies-threaten-global-tobacco-control-progress/

Trofor, A. C., Papadakis, S., Lotrean, L. M., Radu-Loghin, C., Eremia, M., Mihaltan, F., Driezen, P., Kyriakos, C. N., Mons, U., Demjén, T., Nogueira, S. O., Fernández, E., Tountas, Y., Przewoźniak, K., McNeill, A., Fong, G. T., & Vardavas, C. I. (2018). Knowledge of the health risks of smoking and impact of cigarette warning labels among tobacco users in six European countries: Findings from the EUREST-PLUS ITC Europe Surveys. Tobacco Induced Diseases, 16(October), 1–13. https://doi.org/10.18332/tid/99542 DOI: https://doi.org/10.18332/tid/99542

Tsai, J. C., Chen, Y. A., Wu, J. T., Cheng, K. C., Lai, P. S., Liu, K. F., Lin, Y. K., Huang, Y. T., & Hsieh, C. W. (2019). Extracts from fermented black garlic exhibit a hepatoprotective effect on acute hepatic injury. Molecules, 24(6), 1–13. https://doi.org/10.3390/molecules24061112 DOI: https://doi.org/10.3390/molecules24061112

Wei, Z., Shan, Y., Tao, L., Liu, Y., Zhu, Z., Liu, Z., Wu, Y., Chen, W., Wang, A., & Lu, Y. (2017). Diallyl trisulfides, a natural histone deacetylase inhibitor, attenuate HIF-1α synthesis, and decreases breast cancer metastasis. In Molecular Carcinogenesis (Vol. 56, Issue 10). https://doi.org/10.1002/mc.22686 DOI: https://doi.org/10.1002/mc.22686

Widyanti, A. S., Ardiaria, M., & Widyastuti, N. (2020). Pengaruh pemberian ubi jalar ungu (Ipomoea batatas L.Poir) terhadap kadar superoksida dismutase (SOD) tikus wistar jantan (Rattus Norvegicus) yang dipapar asap rokok. Jurnal Gizi Indonesia, 8(1), 45. https://doi.org/10.14710/jgi.8.1.45-50 DOI: https://doi.org/10.14710/jgi.8.1.45-50

World Health Organization. (2024). Tobacco: E-cigarettes. World Health Organization. https://www.who.int/news-room/questions-and-answers/item/tobacco-e-cigarettes#:~:text=Are ENDS addictive%3F,can reach dangerously high levels).

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Published

2024-06-30

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Anandasari, N. F. A., Kridayanti, N., Kaspirayanthi, N. K. D., Nurseta, T., Raras, T. Y. M., Khotimah, H., … Yudhanto, H. S. (2024). The Effects of Black Garlic on Ovarian Malondialdehyde, Oviduct Muscle, and Endometrial Arterioles in Smoke-Exposed Rats. JURNAL INFO KESEHATAN, 22(2), 244–258. https://doi.org/10.31965/infokes.Vol22.Iss2.1533

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Vol. 22 No. 2 (2024): JURNAL INFO KESEHATAN

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