The Effect of Sub-Acute Inhalation Exposure to Polyethylene and Polyvinyl Chloride Micro-Nano Plastics on the Superoxide Dismutase (SOD) Level and Malondialdehyde (MDA) Level in Rat Ovary
DOI:
https://doi.org/10.31965/infokes.Vol22.Iss3.1612Keywords:
Polyethylene, Polyvinyl Chloride, Oxidative Stress, Rat OvaryAbstract
Plastic is a synthetic or semi-synthetic organic polymer that is widely used in daily life and in industrial production. Microplastics are widespread contaminants and can enter the human body through the consumption of foods containing microplastics, inhalation of microplastics in the air, and skin contact with microplastic particles present in products. Microplastics can enter the ovaries as foreign bodies and can cause inflammation, oxidative stress, and even ovarian granulosa cell death. Polyethylene plastic shards generally have a higher ability to absorb environmental toxins than other types of plastic. Polyvinyl chloride (PVC) is one of the oldest thermoplastic polymers that is often used as water pipes. PVC has carcinogenic monomers and some harmful additives. This study aims to determine the effect of subacute exposure to micro-nanoplastics per inhalation on SOD and MDA levels in rat ovaries. The research method used is a true experimental design with a Randomize Post Test Only Group Design research design. This study used the ovarian organs of female white rats that had been exposed to PVC and PE for 28 days. The number of samples used in this study amounted to 18 female rats. Subacute exposure to micro-nanoplastics per inhalation can lower SOD levels and significantly increase MDA levels in rat ovaries. This study is expected to provide knowledge and an overview for future research on the mechanism of toxicity of micro-nanoplastic exposure that has an impact on female infertility through free radicals in the ovaries.
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An, R., Wang, X., Yang, L., Zhang, J., Wang, N., Xu, F., ... & Zhang, L. (2021). Polystyrene microplastics cause granulosa cells apoptosis and fibrosis in ovary through oxidative stress in rats. Toxicology, 449, 152665. https://doi.org/10.1016/j.tox.2020.152665
Baj, J., Dring, J. C., Czeczelewski, M., Kozyra, P., Forma, A., Flieger, J., ... & Teresiński, G. (2022). Derivatives of plastics as potential carcinogenic factors: The current state of knowledge. Cancers, 14(19), 4637. https://doi.org/10.3390/cancers14194637
Bala, R., Singh, V., Rajender, S., & Singh, K. (2021). Environment, lifestyle, and female infertility. Reproductive sciences, 28, 617-638. https://doi.org/10.1007/s43032-020-00279-3
Blackburn, K., & Green, D. (2022). The potential effects of microplastics on human health: What is known and what is unknown. Ambio, 51(3), 518-530. https://doi.org/10.1007/s13280-021-01589-9
Cary, C. M., Seymore, T. N., Singh, D., Vayas, K. N., Goedken, M. J., Adams, S., ... & Stapleton, P. A. (2023). Single inhalation exposure to polyamide micro and nanoplastic particles impairs vascular dilation without generating pulmonary inflammation in virgin female Sprague Dawley rats. Particle and Fibre Toxicology, 20(1), 16. https://doi.org/10.1186/s12989-023-00525-x
Cheng, Y., Zhu, L., Song, W., Jiang, C., Li, B., Du, Z., ... & Zhang, K. (2020). Combined effects of mulch film-derived microplastics and atrazine on oxidative stress and gene expression in earthworm (Eisenia fetida). Science of the Total Environment, 746, 141280. https://doi.org/10.1016/j.scitotenv.2020.141280
Dhaka, V., Singh, S., Anil, A. G., Sunil Kumar Naik, T. S., Garg, S., Samuel, J., ... & Singh, J. (2022). Occurrence, toxicity and remediation of polyethylene terephthalate plastics. A review. Environmental Chemistry Letters, 20(3):1777–1800. https://doi.org/10.1007/s10311-021-01384-8
Esterhuizen, M., & Kim, Y. J. (2022). Effects of polypropylene, polyvinyl chloride, polyethylene terephthalate, polyurethane, high-density polyethylene, and polystyrene microplastic on Nelumbo nucifera (Lotus) in water and sediment. Environmental Science and Pollution Research, 29(12), 17580-17590. https://doi.org/10.1007/s11356-021-17033-0
Farag, A. A., Youssef, H. S., Sliem, R. E., El Gazzar, W. B., Nabil, N., Mokhtar, M. M., ... & Sayed, A. E. D. H. (2023). Hematological consequences of polyethylene microplastics toxicity in male rats: Oxidative stress, genetic, and epigenetic links. Toxicology, 492, 153545. https://doi.org/10.1016/j.tox.2023.153545.
De Guzman, M. C., Chua, P. A. P., & Sedano, F. S. (2020). Embryotoxic and teratogenic effects of polyethylene microbeads found in facial wash products in Zebrafish (Danio rerio) using the Fish Embryo Acute Toxicity Test. bioRxiv. https://doi.org/10.1101/2020.09.16.299438
Hong, Y., Wu, S., & Wei, G. (2023). Adverse effects of microplastics and nanoplastics on the reproductive system: A comprehensive review of fertility and potential harmful interactions. Science of The Total Environment, 903, 166258. https://doi.org/10.1016/j.scitotenv.2023.166258
Hou, J., Lei, Z., Cui, L., Hou, Y., Yang, L., An, R., ... & Zhang, L. (2021). Polystyrene microplastics lead to pyroptosis and apoptosis of ovarian granulosa cells via NLRP3/Caspase-1 signaling pathway in rats. Ecotoxicology and Environmental Safety, 212, 112012. https://doi.org/10.1016/j.ecoenv.2021.11201
Liu, Y., Zhang, J., Zhao, H., Cai, J., Sultan, Y., Fang, H., ... & Ma, J. (2022). Effects of polyvinyl chloride microplastics on reproduction, oxidative stress and reproduction and detoxification-related genes in Daphnia magna. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 254, 109269. https://doi.org/10.1016/j.cbpc.2022.10926
Liu, Z., Zhuan, Q., Zhang, L., Meng, L., Fu, X., & Hou, Y. (2022). Polystyrene microplastics induced female reproductive toxicity in mice. Journal of hazardous materials, 424, 127629. https://doi.org/10.1016/j.jhazmat.2021.127629
Ma, L., Wu, Z., Lu, Z., Yan, L., Dong, X., Dai, Z., ... & Li, C. (2024). Differences in toxicity induced by the various polymer types of nanoplastics on HepG2 cells. Science of The Total Environment, 918, 170664. https://doi.org/10.1016/j.scitotenv.2024.170664.
Mardiyana & Kristiningsih, A. (2020). Dampak Pencemaran Mikroplastik di Ekosistem Laut terhadap Zooplankton. Jurnal Pengendalian Pencemaran Lingkungan (JPPL), 2(1), 29-36. https://doi.org/10.35970/jppl.v2i1.147
PlasticsEurope .(2019). Plastics—The Facts 2019. An Analysis of European Plastics Production, Demand and Waste Data. PlasticEurope. Retrieved from: https://www.plasticseurope.org/en/resources/publications/1804-plastics-facts-2019
Prata, J. C., da Costa, J. P., Lopes, I., Duarte, A. C., & Rocha-Santos, T. (2020). Environmental exposure to microplastics: An overview on possible human health effects. Science of the total environment, 702, 134455. https://doi.org/10.1016/j.scitotenv.2019.134455
Prokić, M. D., Radovanović, T. B., Gavrić, J. P., & Faggio, C. (2019). Ecotoxicological effects of microplastics: Examination of biomarkers, current state and future perspectives. TrAC Trends in analytical chemistry, 111, 37-46. https://doi.org/10.1016/j.trac.2018.12.001
Safitriana, S. (2022). Kemandulan (Infertil) Stigma Negatif Pada Wanita Indonesia. Jakarta: Kemenkes Direktorat Jenderal Pelayanan Kesehatan. Retrieved from: https://yankes.kemkes.go.id/view_artikel/12/kemandulan-infertil-stigma-negatif-pada-wanita-indonesia.
Sincihu, Y., Keman, S., Steven, S., Jaya, D. P., Wicaksono, L. S., Palyama, P. N., ... & Supit, V. (2022). Dampak Pemberian Mikroplastik Poliethilen Peroral Terhadap Hitung Jenis Sel Leukosit Darah Rattus Norvegicus Strain Wistar. Dampak Pemberian Mikroplastik Poliethilen Peroral Terhadap Hitung Jenis Sel Leukosit Darah Rattus Norvegicus Strain Wistar, 6(1), 1-10.
Triananda, A. S., Primadiamanti, A., & Angin, M. P. (2023). Uji Antioksidan Ekstrak Etanol Batang Pepaya (Carica papaya L) Dengan Pengukuran Kadar Malondialdehid (MDA) Menggunakan Metode Spektrofotometer Uv-Vis Pada Mencit. Analit: Analytical and Environmental Chemistry, 45-55. https://doi.org/10.23960/aec.v8i1.2023.p45-55
Trivantira, N. S., Fitriyah, F., & Ahmad, M. (2023). Identifikasi Jenis Polimer Mikroplastik Pada Ikan Tongkol Lisong (Auxis Rochei) Di Pantai Damas Prigi Kabupaten Trenggalek Jawa Timur. Biology Natural Resources Journal, 2(1), 19-23. https://doi.org/10.55719/Binar.2023.2.1.19-23
Walker, Matthew H., and Kyle J. Tobler. 2022. Female Infertility. StatPearls Publishing.
Wang, J., Li, Y., Lu, L., Zheng, M., Zhang, X., Tian, H., ... & Ru, S. (2019). Polystyrene microplastics cause tissue damages, sex-specific reproductive disruption and transgenerational effects in marine medaka (Oryzias melastigma). Environmental Pollution, 254, 113024. https://doi.org/10.1016/j.envpol.2019.113024
WHO. (2023). Infertility. Geneva: WHO. Retrieved: https://www.who.int/news-room/fact-sheets/detail/infertility.
Yang, S., Li, M., Kong, R. Y. C., Li, L., Li, R., Chen, J., & Lai, K. P. (2023). Reproductive toxicity of micro-and nanoplastics. Environment International, 177, 108002. https://doi.org/10.1016/j.envint.2023.108002.
Yang, W., Jannatun, N., Zeng, Y., Liu, T., Zhang, G., Chen, C., & Li, Y. (2022). Impacts of microplastics on immunity. Frontiers in toxicology, 4, 956885. https://doi.org/10.3389/ftox.2022.956885
Zheng, M., Liu, Y., Zhang, G., Yang, Z., Xu, W., & Chen, Q. (2023). The applications and mechanisms of superoxide dismutase in medicine, food, and cosmetics. Antioxidants, 12(9), 1675. https://doi.org/10.3390/antiox12091675
Zhou, Y., Jin, Q., Xu, H., Wang, Y., & Li, M. (2023). Chronic nanoplastic exposure induced oxidative and immune stress in medaka gonad. Science of The Total Environment, 869, 161838. https://doi.org/10.1016/j.scitotenv.2023.161838.
Zientika, Z., Amin, B., & Yoswaty, D. (2021). Relationship Between Microplastics Abundance and Sediment Organic Content in Dumai Coastal Waters. Journal of Coastal and Ocean Sciences, 2(3), 154-159. https://doi.org/10.31258/jocos.2.3.154-159.
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