The Effectiveness of Chocolate in Reducing the Number of Methicillin-Resistant Staphylococcus aureus Colonies in Rattus norvegicus

Authors

  • Edy Suwandi Department of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak, Pontianak, West Kalimantan, Indonesia.
  • Ari Nuswantoro Department of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak, Pontianak, West Kalimantan, Indonesia. https://orcid.org/0000-0001-9525-393X
  • Sugito Sugito Department of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak, Pontianak, West Kalimantan, Indonesia.
  • Desi Wahyumarniasari Department of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak, Pontianak, West Kalimantan, Indonesia.
  • Muhammad Reza Setiawan Department of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak, Pontianak, West Kalimantan, Indonesia.
  • Dinasti Aprillia Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak, Pontianak, West Kalimantan, Indonesia.
  • Devi Nurfitri Bintang Department of Pharmacy, Akademi Farmasi Yarsi Pontianak, Pontianak, West Kalimantan, Indonesia.

DOI:

https://doi.org/10.31965/infokes.Vol19.Iss2.545

Keywords:

Chocolate, Prebiotics, Probiotics, Methicillin-resistant Staphylococcus aureus

Abstract

Chocolate has long been understood to provide positive emotions and a good mood if consumed in moderation. Chocolate contains prebiotics naturally from its constituent ingredients produced during the production process. Prebiotics, frequently oligosaccharides, are substances which cannot be metabolized by the human digestive system but can be employed by a group of bacteria in the gut, understood as probiotics. The positive relationship among them provides benefits for the host in eliminating pathogens. One of the well-known pathogens which frequently cause infection either in the community or in hospitals is methicillin-resistant Staphylococcus aureus (MRSA). Since it was first identified in 1960, MRSA has caused health problems until today. Research conducted on two groups of Rattus norvegicus infected with MRSA and then fed chocolate revealed a decrease in the average number of bacterial colonies on the skin compared to the control group. In the group fed chocolate at a dose of 50 mg/day, the bacterial colonies decreased to 1.28 x 108 CFU/cm2 in 7 days, lower than in the control group (1.46 x 108 CFU/cm2) at the same time. While those fed 75 mg/day chocolate decreased to 2.70 x 107 CFU/cm2 and the three groups were significantly different (0.000<0.05). Prebiotics fermented by probiotics release short-chain fatty acids (SCFA), which compete with the pathogens for attaching to the epithelial wall so that pathogens lose space and nutrients to survive. However, the adverse effect of chocolate may occur because it contains sugar which is a nutrient for bacteria, but if the balance of normal flora and adequate intake of prebiotics are administered, the pathogen could be eliminated.

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References

Ahmad, A., & Khalid, S. (2018). Therapeutic Aspects of Probiotics and Prebiotics. Diet, Microbiome and Health, 53–91. doi: https://doi.org/10.1016/B978-0-12-811440-7.00003-X DOI: https://doi.org/10.1016/B978-0-12-811440-7.00003-X

Al-Sheraji, S. H., Ismail, A., Manap, M. Y., Mustafa, S., Yusof, R. M., & Hassan, F. A. (2013). Prebiotics as functional foods: A review. Journal of Functional Foods, 5(4), 1542–1553. doi: https://doi.org/10.1016/J.JFF.2013.08.009 DOI: https://doi.org/10.1016/j.jff.2013.08.009

Aryal, S. (2020). McFarland Standards- Principle, Preparation, Uses, Limitations. https://microbenotes.com/mcfarland-standards/

Barišić, V., Kopjar, M., Jozinović, A., Flanjak, I., Ačkar, Đ., Miličević, B., ... & Babić, J. (2019). The chemistry behind chocolate production. Molecules, 24(17), 3163. doi: https://doi.org/10.3390/molecules24173163 DOI: https://doi.org/10.3390/molecules24173163

Ejtahed, H. S., Mohtadi Nia, J., Homayouni Rad, A., Niafar, M., Asghari Jafarabadi, M., & Mofid, V. (2011). The Effects of Probiotic and Conventional Yoghurt on Diabetes Markers and Insulin Resistance in Type 2 Diabetic Patients: A Randomized Controlled Clinical Trial. Iranian Journal of Endocrinology and Metabolism, 13(1), 1–8. Retrieved from: http://ijem.sbmu.ac.ir/browse.php?a_code=A-10-932-1&slc_lang=en&sid=1

El-Hack, M. E. A., El-Saadony, M. T., Shafi, M. E., Alshahrani, O. A., Saghir, S. A. M., Al-wajeeh, A. S., Al-shargi, O. Y. A., Taha, A. E., Mesalam, N. M., & Abdel-Moneim, A.-M. E. (2021). Prebiotics can restrict Salmonella populations in poultry: a review. Animal Biotechnology, 32(5), 1–10. doi: https://doi.org/10.1080/10495398.2021.1883637 DOI: https://doi.org/10.1080/10495398.2021.1883637

El-Hadedy, D., & Abu El-Nour, S. (2012). Identification of Staphylococcus aureus and Escherichia coli isolated from Egyptian food by conventional and molecular methods. Journal of Genetic Engineering and Biotechnology, 10(1), 129–135. doi: https://doi.org/10.1016/j.jgeb.2012.01.004 DOI: https://doi.org/10.1016/j.jgeb.2012.01.004

Eor, J. Y., Tan, P. L., Lim, S. M., Choi, D. H., Yoon, S. M., Yang, S. Y., & Kim, S. H. (2019). Laxative effect of probiotic chocolate on loperamide-induced constipation in rats. Food Research International, 116, 1173–1182. doi: https://doi.org/10.1016/j.foodres.2018.09.062 DOI: https://doi.org/10.1016/j.foodres.2018.09.062

Gonzalez, N. J., Adhikari, K., & Sancho-Madriz, M. F. (2011). Sensory characteristics of peach-flavored yogurt drinks containing prebiotics and synbiotics. LWT - Food Science and Technology, 44(1), 158–163. doi: https://doi.org/10.1016/j.lwt.2010.06.008 DOI: https://doi.org/10.1016/j.lwt.2010.06.008

Granato, D., Branco, G. F., Nazzaro, F., Cruz, A. G., & Faria, J. A. F. (2010). Functional Foods and Nondairy Probiotic Food Development: Trends, Concepts, and Products. Comprehensive Reviews in Food Science and Food Safety, 9(3), 292–302. doi: https://doi.org/10.1111/j.1541-4337.2010.00110.x DOI: https://doi.org/10.1111/j.1541-4337.2010.00110.x

Homayouni, A., Azizi, A., Javadi, M., Mahdipour, S., & Ejtahed, H. (2012). Factors influencing probiotic survival in ice cream: a review. International Journal of Dairy Science, 7(1), 1-10.doi: https://doi.org/10.3923/ijds.2012.1.10 DOI: https://doi.org/10.3923/ijds.2012.1.10

Homayouni, A., Payahoo, L., & Azizi, A. (2012). Effects of Probiotics on Lipid Profile: A Review. American Journal of Food Technology, 7(5), 251–265. doi: https://doi.org/10.3923/ajft.2012.251.265 DOI: https://doi.org/10.3923/ajft.2012.251.265

Karska-Wysocki, B., Bazo, M., & Smoragiewicz, W. (2010). Antibacterial activity of Lactobacillus acidophilus and Lactobacillus casei against methicillin-resistant Staphylococcus aureus (MRSA). Microbiological Research, 165(8), 674–686. doi: https://doi.org/10.1016/j.micres.2009.11.008 DOI: https://doi.org/10.1016/j.micres.2009.11.008

Khangwal, I., & Shukla, P. (2019). Prospecting prebiotics, innovative evaluation methods, and their health applications: a review. 3 Biotech, 9(187). doi: https://doi.org/10.1007/s13205-019-1716-6 DOI: https://doi.org/10.1007/s13205-019-1716-6

Konar, N., Toker, O. S., Oba, S., & Sagdic, O. (2016). Improving functionality of chocolate: A review on probiotic, prebiotic, and/or synbiotic characteristics. Trends in Food Science & Technology, 49, 35–44. doi: https://doi.org/10.1016/j.tifs.2016.01.002 DOI: https://doi.org/10.1016/j.tifs.2016.01.002

Lahtinen, S. J., Ouwehand, A. C., Salminen, S. J., Forssell, P., & Myllärinen, P. (2007). Effect of starch- and lipid-based encapsulation on the culturability of two Bifidobacterium longum strains. Letters in Applied Microbiology, 44(5), 500–505. doi: https://doi.org/10.1111/j.1472-765X.2007.02110.x DOI: https://doi.org/10.1111/j.1472-765X.2007.02110.x

Lee, A. S., de Lencastre, H., Garau, J., Kluytmans, J., Malhotra-Kumar, S., Peschel, A., & Harbarth, S. (2018). Methicillin-resistant Staphylococcus aureus. Nature Reviews Disease Primers, 4(1), 18033. doi: https://doi.org/10.1038/nrdp.2018.33 DOI: https://doi.org/10.1038/nrdp.2018.33

Lin, C.-S., Chang, C.-J., Lu, C.-C., Martel, J., Ojcius, D. M., Ko, Y.-F., Young, J. D., & Lai, H.-C. (2014). Impact of the Gut Microbiota, Prebiotics, and Probiotics on Human Health and Disease. Biomedical Journal, 37(5), 259–269. doi: https://doi.org/10.4103/2319-4170.138314 DOI: https://doi.org/10.4103/2319-4170.138314

Lolou, V., & Panayiotidis, M. I. (2019). Functional Role of Probiotics and Prebiotics on Skin Health and Disease. Fermentation, 5(2), 41. doi: https://doi.org/10.3390/fermentation5020041 DOI: https://doi.org/10.3390/fermentation5020041

Morais, E. C., Morais, A. R., Cruz, A. G., & Bolini, H. M. A. (2014). Development of chocolate dairy dessert with addition of prebiotics and replacement of sucrose with different high-intensity sweeteners. Journal of Dairy Science, 97(5), 2600–2609. doi: https://doi.org/10.3168/jds.2013-7603 DOI: https://doi.org/10.3168/jds.2013-7603

Quigley, E. M. (2019). Prebiotics and Probiotics in Digestive Health. Clinical Gastroenterology and Hepatology : The Official Clinical Practice Journal of the American Gastroenterological Association, 17(2), 333–344. doi: https://doi.org/10.1016/j.cgh.2018.09.028 DOI: https://doi.org/10.1016/j.cgh.2018.09.028

Rad, A. H., Akbarzadeh, F., & Mehrabany, E. V. (2012). Which are more important: Prebiotics or probiotics?. Nutrition, 1196–1197. doi: https://doi.org/10.1016/j.nut.2012.03.017 DOI: https://doi.org/10.1016/j.nut.2012.03.017

Rad, A. H., Mehrabany, E. V., Alipoor, B., Mehrabany, L. V., & Javadi, M. (2012). Do probiotics act more efficiently in foods than in supplements?. Nutrition, 28(7/8), 733.–736. doi: https://doi.org/10.1016/j.nut.2012.01.012 DOI: https://doi.org/10.1016/j.nut.2012.01.012

Rad, A. H., Mehrabany, E. V., Alipoor, B., & Mehrabany, L. V. (2016). The Comparison of Food and Supplement as Probiotic Delivery Vehicles. Critical Reviews in Food Science and Nutrition, 56(6), 896–909. https://doi.org/10.1080/10408398.2012.733894 DOI: https://doi.org/10.1080/10408398.2012.733894

Rad, A. H., Azizi, A., Darghahi, R., Bakhtiari, O., Javadi, M., Moghaddam, M. J., ... & Pirouzian, H. R. (2018). Development of synbiotic milk chocolate enriched with Lactobacillus paracasei, D-tagatose and galactooligosaccharide. Applied Food Biotechnology, 5(2), 59-68. doi: https://dx.doi.org/10.22037/afb.v5i2.19955

Rad, A. H., Torab, R., Ghalibaf, M., Norouzi, S., & Mehrabany, E. V. (2013). Might patients with immune-related diseases benefit from probiotics?. Nutrition, 29(3), 583.–586. doi: https://doi.org/10.1016/j.nut.2012.10.008 DOI: https://doi.org/10.1016/j.nut.2012.10.008

Rad, A. H., Torab, R., Mortazavian, A. M., Mehrabany, E. V., & Mehrabany, L. V. (2013). Can probiotics prevent or improve common cold and influenza? Nutrition, 29(5), 805–806. doi: https://doi.org/10.1016/j.nut.2012.10.009 DOI: https://doi.org/10.1016/j.nut.2012.10.009

Ribeiro de Souza da Cunha, M. de L. (2018). Methods for the Identification, Characterization, and Tracking the Spread of Staphylococcus aureus. Staphylococcus Aureus, 105–125. doi: https://doi.org/10.1016/B978-0-12-809671-0.00006-1 DOI: https://doi.org/10.1016/B978-0-12-809671-0.00006-1

Roberfroid, M., Gibson, G. R., Hoyles, L., McCartney, A. L., Rastall, R., Rowland, I., ... & Meheust, A. (2010). Prebiotic effects: metabolic and health benefits. British Journal of Nutrition, 104(S2), S1-S63. doi: https://doi.org/10.1017/S0007114510003363 DOI: https://doi.org/10.1017/S0007114510003363

Sarao, L. K., & Arora, M. (2017). Probiotics, prebiotics, and microencapsulation: A review. Critical Reviews in Food Science and Nutrition, 57(2), 344–371. doi: https://doi.org/10.1080/10408398.2014.887055 DOI: https://doi.org/10.1080/10408398.2014.887055

Scheid, M. M. A., Moreno, Y. M. F., Junior, M. R. M., & Pastore, G. M. (2013). Effect of prebiotics on the health of the elderly. Food research international, 53(1), 426-432. doi: https://doi.org/10.1016/j.foodres.2013.04.003 DOI: https://doi.org/10.1016/j.foodres.2013.04.003

Sikorska, H., & Smoragiewicz, W. (2013). Role of probiotics in the prevention and treatment of meticillin-resistant Staphylococcus aureus infections. International Journal of Antimicrobial Agents, 42(6), 475–481. doi: https://doi.org/10.1016/j.ijantimicag.2013.08.003 DOI: https://doi.org/10.1016/j.ijantimicag.2013.08.003

Slavin, J. (2013). Fiber and prebiotics: mechanisms and health benefits. Nutrients, 5(4), 1417–1435. doi: https://doi.org/10.3390/nu5041417 DOI: https://doi.org/10.3390/nu5041417

Thermo Fisher Scientific. (2001). Dehydrated Culture Media - Plate Count Agar. Thermo Fisher Scientific. Available on: http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0325&c=UK&lang=EN

Turner, N. A., Sharma-Kuinkel, B. K., Maskarinec, S. A., Eichenberger, E. M., Shah, P. P., Carugati, M., ... & Fowler, V. G. (2019). Methicillin-resistant Staphylococcus aureus: an overview of basic and clinical research. Nature Reviews Microbiology, 17(4), 203-218. doi: https://doi.org/10.1038/s41579-018-0147-4 DOI: https://doi.org/10.1038/s41579-018-0147-4

Vinayak, A., Mudgal, G., Sharma, S., & Singh, G. B. (2021). Prebiotics for Probiotics. Advances in Probiotics for Sustainable Food and Medicine. Singapore: Springer. doi: https://doi.org/10.1007/978-981-15-6795-7_4 DOI: https://doi.org/10.1007/978-981-15-6795-7_4

Wallace, T. C., Guarner, F., Madsen, K., Cabana, M. D., Gibson, G., Hentges, E., & Sanders, M. E. (2011). Human gut microbiota and its relationship to health and disease. Nutrition Reviews, 69(7), 392–403. doi: https://doi.org/10.1111/j.1753-4887.2011.00402.x DOI: https://doi.org/10.1111/j.1753-4887.2011.00402.x

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Published

2021-12-31

How to Cite

Suwandi, E., Nuswantoro, A., Sugito, S., Wahyumarniasari, D., Setiawan, M. R., Aprillia, D., & Bintang, D. N. (2021). The Effectiveness of Chocolate in Reducing the Number of Methicillin-Resistant Staphylococcus aureus Colonies in Rattus norvegicus . JURNAL INFO KESEHATAN, 19(2), 154–161. https://doi.org/10.31965/infokes.Vol19.Iss2.545

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Vol. 19 No. 2 (2021): JURNAL INFO KESEHATAN

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