Identifikasi Bakteri Gram Negatif Galur Extended Spectrum Beta Lactamase Pada Ruang NICU RSUD Prof. DR. W. Z. Johannes Kupang
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Extended Spectrum Beta-Lactamase (ESBL) is an enzyme that is capable of hydrolyzing antibiotics from penicillin groups, the generation I, II, III cephalosporins, and monobactam. ESBL is most commonly isolated from Enterobacteriaceae, especially Escherichia coli and Klebsiella pneumonia. The spread of ESBL-producing Enterobacteriaceae can also occur due to mutations. Cases of ESBL detection in hospitals have been widely reported throughout the world including Indonesia. The purpose of this study was to identify Enterobacteriaceae which included ESBL strains isolated from the NICU room of RSUD Prof. Dr. W. Z. Johannes Kupang in 2015. This study was a descriptive study with a cross-sectional approach. The samples used were 18 swab specimens from room facilities collected by accidental sampling method. Swab specimens are grown in Blood Agar Plate and Mac Conkey Agar. Bacterial identification methods are equipped with microscopic tests and biochemical tests. Klebsiella sp was identified and then followed by the antimicrobial sensitivity test (Kirby Bauer) against ceftazidime and ceftriaxone. ESBL confirmation test uses the Double Disc Sinergy Test (DDST) method. The results of the antibiotic sensitivity test showed Klebsiella sp. resistant to the antibiotics ceftazidime and ceftriaxone. DDST test shows ESBL production from Klebsiella sp. It was concluded that ESBL-producing Enterobacteriaceae found were Klebsiella sp which had shown resistance to third-generation cephalosporins (Ceftazidime and Ceftriaxone).
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Al-Zahrani, A.J., and Akhtar, N. 2005. Susceptibility Patterns of Extended Spectrum ß-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Isolated in a Teaching Hospital. Departement of Microbiology, College of Medicine, King Faisal University, Dammam, Saudi Arabia. Pakistan J. Med. Res. Vol. 44, No 2
Asrining Surami.2003. Perawatan bayi risiko tinggi. Jakarta: EGC.
Brooks JF, Carrol CV, Butel JS, Morse SA.2007. Jawetz melnick & adelbergs Medical Microbiology .24th ed. San Fransisko: McGraw-Hill Companies.
Brunton LL, Goodman & Gilman’s. 2006. The pharmacological Basis of Therapeutics.11th ed. McGraw-Hill.
Bush, K., Jacoby, G.A.2010. Updated Functional Classification of β- Lactamases. Antimicrob. Agents Chemother. 54:3:969-976.
Cappucino, J.G., and N. Sherman.2005. Microbiology: A Laboratory Manual. The Benjamin/Cummings Publishing Company Inc. California USA. P.127-148
Carol.A .2007. Firs Generation of Cephalosporine: summary of the first international symposium. J Antimicrob Chemother. Clin Microbiol Rev; 14:933-951
CLSI M100-S24 - Performance Standards for Antimicrobial Susceptibility Testing, 2014.
Connie et al.2011. Textbook of Diagnostic Microbiologi. fourth edition.W.B. Saunder company
Danny Luhulima. 2011. Aspek Laboratorium Extended Spectrum Beta Lactamase. Patologi Klinik Fakultas Kedokteran Universitas Airlangga
David L. Paterson, Robert A. Bonomo. 2005. Extended-Spectrum β-lactamase: Clinical Update. American Society for Microbiology
Dakh F. Mutation frequency of non ESBL phenotype SENTRY (AsiaPacific) Isolates of Klebsiella Pneumoniae Conversion to an ESBL Positive Phenotype. Queensland University of Technology School of Life Sciences. 2008:305-11
Duttaroy B, Mehta S .2005. Extended spectrum β-lactamases (ESBL) in clinical isolates of Klebsiella pneumoniae and Escherichia coli. Indian J. Pathol. Microbiol., 48(1): 45-48
Elena, S.F., Whittam, T. S., Winkworth C. L., Riley M. A., and Lenski, R.E., 2005. Genomic Divergence of Escherichia coli strains: Evidence for Horizontal Transfer and Variation in Mutation Rates. International Microbiology 8:271-78.
Erin. 2014. Kualitas Mikrobiologi Udara di Inkubator Unit Perinatologi RSUD Dr. Abdul Moeloek Bandar Lampung. Ilmu Kesehatan Anak Fakultas Kedokteran Universitas Lampung
EUCAST guidelines for detection of resistance mechanisms and specific resistances of clinical and/or epidemiological importance. Version 1.0, 2013.
Goodman & Gilman. 2008. Dasar Farmakologi terapi. Penerbit Buku Kedokteran. Jakarta: EGC.
Gunawan SG, Setiabudy R, Nafrialdi, Elisabeth. 2007. Farmakologi dan terapi 5th ed. Jakarta: Balai penerbit FKUI.
FKUI. 2009. Disentri Ilmu Kesehatan Anak. Jilid II. Cetakan ke-II. Jakarta
Fuda CCS, Fisher JF, Mobasherry A. Betalactam resitance S. aureus the adaptive resistance plasmid genome. Cellu ler and Mol eculer life Sciences, 2005; p 215-9
Istiantoro, yati H dan Gan, Vincent HS. Penisilin, Sefalosporin dan betalaktam lainnya. Dalam: Ganiswara, Sulista G, editor. Farmakologi dan terapi. Edisi 5. Jakarta: Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia: 2007. Hal. 664-93
Jawetz. 2005. Mikrobiologi Kedokteran Jawetz, melnick and adelberg, Ed.23, Translation of Jawetz, Melnick and adelberg Medical Microbiologi, 23thEd. Alih Bahasa oleh Hartanto, H., et al. Jakarta: EGC
Jeyamohan, Dharsini. Angka prevalensi infeksi nosokomial pada pasien luka operasi pasca bedah di Rumah Sakit Umum Pusat Haji Adam Malik, Medan dari bulan April sampai September 2010. Universitas Sumatera Utara
Waluyo Lud. 2010. Teknik metode dasar dalam Mikrobiologi. Universitas Muhamadiayah Malang
Kemenkes RI, 2011. Peraturan Menteri Kesehatan Republik Indonesia Nomor 2406/Menkes/Per/Xii/2011. Tentang Pedoman Umum Penggunaan Antibiotik
Kurniawan A, Triratna S, Riyanto D, Theodorus. Angka kejadian dan pola kuman infeksi nosokomial pada penderita di ruang perawatan intensif anak RSMH Palembang. JKK. 2009;41:2686-94
Lamont RJ, Burne RA, Lantz MS, Leblanc DJ. Oral Microbilology and Immunology. ASM Press Washington, 2006.p 415-9.
Mayasari, E. 2005. Pseudomonas aaeruginosa: Karakteristik, Infeksi danPenanganan. Medan: Departemen Mikrobiologi Fakultas Kedokteran Universitas Sumatera Utara. Hal. 4-12
Melzer, M. and I. Perersen, 2007. Mortality following bacteraemic infection caused byExtended Spectrum ß Lactamases (ESBL) producing Escherichia coli compared to non ESBL producing Escherichia coli. J infect., SS: 254-259
Maurizio S.2011.Characterization of Clinical Isolates of Enterobacteriaceae from Italy by the BD Phoenix Extended - Spectrum beta - Lactamase Detection Method
Mitchell J. 2010. Utility of NCCLS Guidelines for Identifying Extended-Spectrum beta Lactamases in Non Escherichia coli and Non Klebsiella sp. of Enterobacteriaceae Ni Putu Aryadnyani.2012. Peningkatan Waktu Fermentasi Kombucha Tea Meningkatkan Daya Hambat Pertumbuhan Bakteri Escherichia Coli Penghasil Extended Spectrum Beta Lactamases (Esbl) Secara In Vitro. Program Pascasarjana Universitas Udayana: Denpasar
Norman, L. 2005. Biochemical Test for Identifying Unknowns. MCB 2010 Course Website.http://web.fccj.edu/~lnorman/unknowns.htm?index=2#top [diakses Juli 2015].
Guidelines on susceptibility of antibiotic resistant Enterobacteriaceae due to extended spectrum β-lactamases (ESBL). Canadian External Quality Assurance Advisory Groups on Antimicrobial Resistance (CEQA- AGAR) and Bureau of Microbiology; Health Canada, December 1999
Harley-Prescott. 2002. Laboratory Exercises in Microbiology. Fifth Edition. The Mcgraw-Hill Companies.
Pelczar, M.J. and E. C. S. Chan.2005. Dasar-dasar Mikrobiologi, Jilid 1. Ratna Siri Hadioetomo, Teja Imas, S. Sutarmi jitrosomo, Sri Lestari Angka, penerjemah. Jakarta: Penerbit Universitas Indonesia. Hal.81-91
Peterson DL, Bronomo RA. Extended Spectrum Beta LactamaseL: clinical
update. ClinMycribiol Rev.2005: 18(4): p 657-86
Pratiwi, S.T., 2008, Mikrobiologi Farmasi, 190-192, Jakarta, Erlangga.
Radji, M. 2010. Buku Ajar Mikrobiologi Panduan Mahasiswa Farmasi dan Kedokteran. Jakarta: EGC.
RSIA Bunda.NICU/PICU. http://www.bunda.co.id/rsiabundajakarta/nicu.php. Diakses pada tanggal 10 mei 2015.
Serefhanoglu, K., Turan, H., Timurkaynak, F.E., and Arsian, H. 2009. Bloodstream Infections Caused by ESBL-Producing E. coli and K. pneumonia: Risk n Factors for Multidrug-Resistance. Baskent University, Medical Faculty, Departement of Infectious Diseases and Clinical Microbiology, Ankara/Turkey. The Brazilian Journal of Infectious Diseases and Contexto Publishing. All Rights Reserved.
Sartika. 2013. Isolasi Staphylococcus aureus pada mukosa hidung perokok dan bukan perokok pada buruh di makasar. Fakultas kedokteran universtas hasanudin Makasar
Sharma J, Meera S, Palap R. 2009. Detection of TEM and SHV in Escherichia Coli and Klabisella Pneumonia Isolates in a Tertiary Care Hospital from India. Indian Journal Med Res. 132: 332-336
Sianturi P, Hasibuan B, Lubis B, Azlin E, Tjipta G. Gambaran pola resistensi bakteri di unit perawatan neonates. Sari Pediatri. 2013;13:431-6
Wilianti.2009. Rasionalitas Penggunaan Antibiotik Pada Pasien Infeksi Salurun Kemih Pada Bangsal Penyakit Dalam di RSUD DR. Kariadi Semarang. Karya Tulis Ilmiah. Fakultas Kedokteran Universitas Diponegoro, Semarang
Winarto. Prevalensi Kuman ESBL (Extended Spectrum Beta Lactamase) dari Material Darah di RSUP Dr. Kariadi Tahun 2004-2005. Semarang: Media Medika Indonesia. Fakultas Kedokteran Universitas Diponegoro. 2009; 260-267
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